Subcellular accumulation of different concentrations of cadmium, nickel, and copper in Indian mustard and application of a sigmoidal model.
Subcellular accumulation of different concentrations of cadmium, nickel, and copper in Indian mustard and application of a sigmoidal model.
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DOI：
10.2134/jeq2012.0362
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发表时间：
2013-07
影响因子：
2.4
通讯作者：
Yuanpeng Wang;Jing Huang;Yanzheng Gao
中科院分区：
文献类型：
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作者：
Yuanpeng Wang;Jing Huang;Yanzheng Gao
某些植物物种可以耐受甚至解毒金属元素。这种解毒能力已被证明取决于植物如何在其组织中分配金属元素。反过来，这种分布可能对植物修复产生重要影响。本文研究了不同浓度（10、50、100和300 μmol L）的镉（Cd）、镍（Ni）和铜（Cu）对L.变种（印度芥末）。在300 μmol/L浓度下，Cu和Cd分别有98%和79%滞留在根中，而Ni则分布在叶、茎和根之间，分别占32%、29%和39%。细胞壁和可溶性组分是Cd和Cu在茎和叶中的主要贮藏室，而可溶性组分是Ni在茎和叶中的主要贮藏室。扫描电子显微镜（SEM）X-射线显微照片显示，在300 μmol L的Cd、Ni和Cu处理后，表皮、栅栏和海绵薄壁细胞被破坏。A S形模型是一个强大的工具来描述亚细胞积累和组织浓度的镉，镍，铜暴露于各种浓度的金属。描述蓄积的决定系数（）值较高，但膜亚细胞部分不符合方程。该模型能较好地描述重金属的最大富集量和临界浓度。结果表明，使用的S形模型是一个很有前途的方法，可以提高金属积累在亚细胞水平的印度芥菜的理解。
Certain plant species can tolerate and even detoxify metallic elements. This detoxification ability has been shown to depend on how plants distribute the metallic elements in their tissues. In turn, this distribution may have an important impact on phytoremediation. Here, we investigated the effects of different single-metal concentrations (10, 50, 100, and 300 μmol L) of cadmium (Cd), nickel (Ni), and copper (Cu) on their subcellular distribution in L. var. (Indian mustard). At the applied concentration of 300 μmol L, 98% of the Cu and 79% of Cd were retained in the roots, while Ni was distributed between leaves (32%), stems (29%), and roots (39%). The cell wall and soluble fractions were the dominant storage compartments for Cd and Cu in the stems and leaves, whereas the soluble fraction was the dominant storage compartment for Ni in stems and leaves. Scanning electron microscopy (SEM) X-ray micrographs showed a breakdown of epidermal, palisade, and spongy parenchyma cells following the 300 μmol L Cd, Ni, and Cu treatment. A sigmoidal model is a powerful tool to describe the subcellular accumulation and tissue concentrations of Cd, Ni, and Cu following exposure to various concentrations of metals. The coefficients of determination () values for the description of the accumulation were high, although the membrane subcellular fraction did not fit the equation. Both the maximum accumulation capacity and the critical concentrations of metals were well described by the model. The results indicate that the use of a sigmoidal model is a promising method that could improve the understanding of metal accumulation at the subcellular level in Indian mustard.