ALTERATIONS IN THE HUMAN IMMUNE-RESPONSE TO THE HEPATITIS-B VACCINE AMONG THE ELDERLY
ALTERATIONS IN THE HUMAN IMMUNE-RESPONSE TO THE HEPATITIS-B VACCINE AMONG THE ELDERLY
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DOI：
10.1016/0008-8749(87)90294-2
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发表时间：
1987-10-01
影响因子：
4.3
通讯作者：
RATINAUD, MH
中科院分区：
文献类型：
--
作者：
COOK, JM;GUALDE, N;RATINAUD, MH
本文探讨了B型肝炎疫苗免疫后老年人淋巴细胞与B型肝炎（HBs）抗原的特异性结合。为此，将HBs抗原与荧光微球结合，并使标记的抗原与来自HBs疫苗应答或无应答者的淋巴细胞反应。检测了10例应答者和14例无应答者的淋巴细胞的抗原结合能力。对于对照，将淋巴细胞与含有人白蛋白的微球一起孵育。10个应答者中有8个的淋巴细胞能够识别HBs抗原;对于无应答者，该比率为14个中的9个。结合淋巴细胞是B细胞，而不是T淋巴细胞。将应答者和非应答者的B和T细胞合并，并在存在HBs抗原的情况下培养8天，并计数抗体产生细胞。无论是单独的B细胞还是来自无应答者的B细胞加T细胞都不能产生抗体。另一方面，来自无应答老年人的B细胞在存在HBs抗原和来自应答老年人的T细胞的情况下培养时产生抗体。这些数据表明，一些体内接种乙肝疫苗后不产生抗体的老年人确实具有产生抗体的细胞。这些人不是免疫系统有缺陷，而是免疫功能紊乱。
The specific binding of hepatitis B (HBs) antigen by lymphocytes from old people immunized with hepatitis B vaccine was explored. For that purpose HBs antigen was combined with fluorescent microspheres, and labeled antigen was allowed to react with lymphocytes from HBs vaccine-responsive or unresponsive people. Lymphocytes from 10 responders and 14 nonresponders were tested for their antigen-binding ability. For controls, lymphocytes were incubated with microspheres bearing human albumin. Lymphocytes from 8 out of 10 responders were able to recognize HBs antigen; for the nonresponders the ratio was 9 out of 14. HBs-binding lymphocytes were B cells but not T lymphocytes. B and T cells from responders and nonresponders were combined and cultivated for 8 days in the presence of HBs antigen, and antibody-producing cells were counted. Neither B cells alone or B cells plus T cells from nonresponders were able to produce antibody. On the other hand B cells from unresponsive old people produced antibodies when they were cultivated in the presence of HBs antigen and T cells from responsive old people. These data suggest that some elderly individuals who do not produce antibody after in vivo immunization by HBs vaccine do have antibody-producing cells. Instead of a gap in their immune repertoire, these people are suffering from immune dysfunction.