Growth-inhibiting effects of Coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria
Growth-inhibiting effects of Coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria
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DOI：
10.1021/jf980991o
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发表时间：
1999-03-01
影响因子：
6.1
通讯作者：
Ahn, YJ
中科院分区：
文献类型：
--
作者：
Chae, SH;Jeong, IH;Ahn, YJ
采用浸渍纸片法检测了黄连根提取物对8种人体肠道细菌的生长抑制活性，并与4种市售异喹啉生物碱[硫酸小檗碱（BS）、碘化小檗碱（BI）、氯化巴马汀（PC）和硫酸巴马汀（PS）]以及茶叶提取物表没食子儿茶素没食子酸酯（EGCG）进行了比较。光谱分析表明，黄连提取物的生物活性成分为异喹啉生物碱氯化小檗碱（BC）、碘化巴马汀（PI）和氯化黄连碱（CC）。生长反应随所用的化学品和细菌菌株而变化。在使用500 μ g/片的试验中，BC和PI对长双歧杆菌、两歧双歧杆菌、产气荚膜梭菌和副营养梭菌产生明显的抑制作用，而在双歧杆菌、嗜酸乳杆菌、干酪乳杆菌和大肠杆菌中观察到弱抑制或无抑制作用。在1000 μ g/片时，CC对所有试验细菌显示出弱的生长抑制或没有生长抑制，而EGCG仅对C.产气荚膜梭菌和C.副营养素在各种异喹啉类生物碱中，BC对C.与BI和BS相比，PI对产气荚膜杆菌的抑制作用更明显，而与PC和PS相比，PI对产气荚膜杆菌的抑制作用更明显。黄连提取物对B的生长没有促进作用。longum和C.产气荚膜杆菌
The growth-inhibiting activity of Coptis japonica (Makino) root-derived materials toward eight human intestinal bacteria was examined using an impregnated paper disk method and compared to that of four commercially available isoquinoline alkaloids [berberine sulfate (BS), berberine iodide (BI), palmatine chloride (PC), and palmatine sulfate(PS)], as well as that of Thea sinensis leaf-derived epigallocatechin gallate (EGCG). The biologically active constituents of the Coptis extract were characterized as the isoquinoline alkaloids berberine chloride (BC), palmatine iodide (PI), and coptisine chloride (CC) by spectral analysis. The growth responses varied with both chemical and bacterial strain used. In a test using 500 mu g/disk, BC and PI produced a clear inhibitory effect against Bifidobacterium longum, Bifidobacterium bifidum, Clostridium perfringens, and Clostridium paraputrificum, whereas weak or no inhibition was observed in Bifidobacterium adolescentis, Lactobacillus acidophilus, Lactobacillus casei, and Escherichia coli. At 1000 mu g/disk, CC revealed weak or no growth inhibition toward all test bacteria, whereas EGCG exhibited weak growth inhibition against only C. perfringens and C. paraputrificum. Among various isoquinoline alkaloids, BC exhibited more potent-inhibitory activity toward C. perfringens than BI and BS, whereas the inhibitory effect was more pronounced in PI compared to PC and PS. The Coptis root-derived materials did not promote growth of B. longum and C. perfringens.