miR-153 Silencing Induces Apoptosis in the MDA-MB-231 Breast Cancer Cell Line
miR-153 Silencing Induces Apoptosis in the MDA-MB-231 Breast Cancer Cell Line
复制标题
DOI：
10.7314/apjcp.2013.14.5.2983
复制
发表时间：
2013-01-01
影响因子：
--
通讯作者：
Martin Perez-Santos, Jose Luis
中科院分区：
文献类型：
--
作者：
Anaya-Ruiz, Maricruz;Cebada, Jorge;Martin Perez-Santos, Jose Luis
微小RNA（miRNAs）是小的非编码RNA（18-25个核苷酸），其通过负调节其靶mRNA的稳定性或翻译效率来转录后调节基因表达。在此背景下，本研究旨在评估miR-153抑制在乳腺癌细胞系MDA-MB-231中的体外作用。在用miR-153抑制剂转染MDA-MB-231细胞后48小时，利用MTT（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物）测定来确定miR-153对细胞活力的影响。采用流式细胞仪检测miR-153对MDA-MB-231细胞增殖和凋亡的影响，并检测caspase 3/7活性。我们的结果表明，与对照组相比，miR-153的沉默在48小时时显著抑制生长，使增殖降低37.6%，并诱导凋亡。进一步的研究是必要的，以证实我们的发现，并检查这种microRNA在未来的诊断和治疗干预的潜在用途。
MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-153 inhibition in the breast carcinoma cell line MDA-MB-231. Forty-eight hours after MDA-MB-231 cells were transfected with the miR-153 inhibitor, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects of miR-153 on cell viability. Flow cytometry analysis and assessment of caspase 3/7 activity were adopted to determine whether miR-153 affects the proliferation rates and apoptosis levels of MDA-MB-231 cells. Our results showed that silencing of miR-153 significantly inhibited growth when compared to controls at 48 hours, reducing proliferation by 37.6%, and inducing apoptosis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future diagnostic and therapeutic interventions.