Stabilization of membranes in human platelets freeze-dried with trehalose
Stabilization of membranes in human platelets freeze-dried with trehalose
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DOI：
10.1016/s0009-3084(02)00177-9
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发表时间：
2003-01-01
影响因子：
3.4
通讯作者：
Ricker, J
中科院分区：
文献类型：
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作者：
Crowe, JH;Tablin, F;Ricker, J
人血小板通常在血库中储存3-5天，之后将其丢弃。我们已经开始努力开发长期保存血小板的方法。在以前的研究中，我们已经表明海藻糖可以用于在干燥过程中保护生物膜和蛋白质，并提供了有关机制的证据。关于海藻糖的特殊性质已经形成了一个神话，我们在这里讨论并澄清一些事实和误解。我们已经发现了一种将这种糖引入血小板细胞质的简单方法，并成功地冷冻干燥了负载海藻糖的血小板，结果非常有希望。我们目前的证据表明，膜微区保持完整的血小板与海藻糖冷冻干燥。最后，我们提出了一种可能的机制，通过该机制，微畴被保存。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Human blood platelets are normally stored in blood banks for 3-5 days, after which they are discarded. We have launched an effort at developing means for preserving the platelets for long term storage. In previous studies we have shown that trehalose can be used to preserve biological membranes and proteins during drying and have provided evidence concerning the mechanism. A myth has grown up about special properties of trehalose, which we discuss here and clarify some of what is fact and what is misconception. We have found a simple way of introducing this sugar into the cytoplasm of platelets and have successfully freeze-dried the trehalose-loaded platelets, with very promising results. We present evidence that membrane microdomains are maintained intact in the platelets freeze-dried with trehalose. Finally, we propose a possible mechanism by which the microdomains are preserved. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.