Electrical properties of the nexal membrane studied in rat ventricular cell pairs.
Electrical properties of the nexal membrane studied in rat ventricular cell pairs.
复制标题
在大鼠心室细胞对中研究的神经膜的电特性。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
R. Weingart
中科院分区：
文献类型：
--
作者：
R. Weingart
从成年大鼠心室分离的细胞对被用来表征位于细胞之间的nexal膜的电特性。将细胞对中的每个细胞连接到吸移管，以便能够进行全细胞记录。采用双电压钳方法，其允许控制跨nexal膜的电压梯度。发现在较宽的跨膜电压范围（+/-50 mV）内，跨膜的电流-电压关系呈线性。测量结果显示，表观nexal膜电阻rn（app）的平均值为3.4 M Ω。考虑到未补偿的串联电阻（接入电阻）的贡献，有效的nexal电阻rn（eff）约为1.7 M Ω。发现nexal膜电阻对肌膜电位Vm不敏感（测试电压范围：-90 mV至+30 mV）。nexal膜没有表现出整流特性，即它允许双向脉冲传输同样良好。nexal膜的连接体未表现出时间依赖性门控行为（研究的时间范围：0.1 - 10 s）。
Cell pairs isolated from adult rat ventricles were used to characterize the electrical properties of the nexal membrane located between the cells. Each cell of a cell pair was connected to a suction pipette so as to enable whole‐cell recordings. A double voltage‐clamp method was employed which allowed the voltage gradient across the nexal membrane to be controlled. The current‐voltage relationship of the nexal membrane was found to be linear over a broad range of transnexal voltages ( +/‐ 50 mV). The measurements revealed a mean value for the apparent nexal membrane resistance, rn(app), of 3.4 M omega. Taking into account the contribution of an uncompensated series resistance (access resistance), the effective nexal resistance, rn(eff), amounts to 1.7 M omega, approximately. The nexal membrane resistance was found to be insensitive to the sarcolemmal membrane potential, Vm (voltage range tested: ‐90 mV to +30 mV). The nexal membrane showed no rectifying property, i.e. it allows impulse transmission in both directions equally well. The connexons of the nexal membrane exhibited no time‐dependent gating behaviour (time range investigated: 0.1‐10 s).
单只大鼠心肌细胞中的钠电流。
DOI：
10.1113/jphysiol.1981.sp013879
发表时间：
1981
期刊：
The Journal of physiology
影响因子：
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作者：
Brown,AM;Lee,KS;Powell,T
通讯作者：
Powell,T