Development and characterization of 15 novel microsatellite markers from forest musk deer (Moschus berezovskii)
Development and characterization of 15 novel microsatellite markers from forest musk deer (Moschus berezovskii)
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DOI：
10.1007/s10592-007-9362-7
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发表时间：
2008-06
影响因子：
2.2
通讯作者：
Sha Zhao;Xuan Chen;S. Fang;Q. Wan
中科院分区：
文献类型：
--
作者：
Sha Zhao;Xuan Chen;S. Fang;Q. Wan
利用微卫星技术从林麝群体中分离到15个多态性微卫星位点。这些位点表现出高水平的等位基因多样性，在31个个体的群体中每个位点呈现6-14个等位基因。平均观察和预期杂合性分别为0.82和0.68。这些标记的平均多态信息含量值、累积鉴别力、与未知双亲的总排除概率和与未知父本的总排除概率分别为0.78、1.0、0.9999和1.0，表明这套微卫星座位将成为林麝遗传鉴定的有力工具。
A total of 15 polymorphic microsatellite loci were isolated from forest musk deer (Moschus berezovskii). These loci showed high levels of allelic diversity, presenting 6–14 alleles per locus in a group with 31 individuals. The mean observed and expected heterozygosities were 0.82 and 0.68, respectively. The average polymorphic information content value, cumulative discrimination power, overall probability of exclusion with both unknown parents or with only unknown sire for these markers were 0.78, 1.0, 0.9999 and 1.0, respectively, indicating that this suit of microsatellite loci would be a powerful tool for the genetic determination of the forest musk deer.