Topography and Permeability Analyses of Vasculature‐on‐a‐Chip Using Scanning Probe Microscopies
Topography and Permeability Analyses of Vasculature‐on‐a‐Chip Using Scanning Probe Microscopies
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DOI：
10.1002/adhm.202101186
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发表时间：
2021-08
影响因子：
10
通讯作者：
Yuji Nashimoto;Minori Abe;Ryota Fujii;Noriko Taira;Hiroki Ida;Yasufumi Takahashi;K. Ino;J. Ramón‐Azcón;H. Shiku
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文献类型：
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作者：
Yuji Nashimoto;Minori Abe;Ryota Fujii;Noriko Taira;Hiroki Ida;Yasufumi Takahashi;K. Ino;J. Ramón‐Azcón;H. Shiku
微生理系统（MPS）或器官芯片（OoC）可以在体外模拟器官的生理功能，是在临床前研究中确定人体药物反应的有效工具。然而，MPS的分析严重依赖于光学工具，导致难以对靶细胞功能进行真实的实时和高空间分辨率成像。在这项研究中，扫描探针显微镜（SPM）作为MPS的分析工具的作用进行了评估。在具有堆叠微通道的典型MPS系统中制作一个进入孔，以将SPM探针插入系统中。对于第一项研究，制备仅由内皮细胞组成的简单血管模型用于SPM分析。在血管系统的构建过程中，定量评价渗透性和局部化学通量的变化。在单细胞水平上对血流刺激后内皮细胞的形态学变化进行成像，以进行形貌分析。最后，适应的渗透性和地形分析，使用SPM的肠血管系统的可能性进一步评估。相信这项研究将铺平道路，原位渗透性测定和结构分析的MPS使用SPM。
Microphysiological systems (MPS) or organs‐on‐chips (OoC) can emulate the physiological functions of organs in vitro and are effective tools for determining human drug responses in preclinical studies. However, the analysis of MPS has relied heavily on optical tools, resulting in difficulties in real‐time and high spatial resolution imaging of the target cell functions. In this study, the role of scanning probe microscopy (SPM) as an analytical tool for MPS is evaluated. An access hole is made in a typical MPS system with stacked microchannels to insert SPM probes into the system. For the first study, a simple vascular model composed of only endothelial cells is prepared for SPM analysis. Changes in permeability and local chemical flux are quantitatively evaluated during the construction of the vascular system. The morphological changes in the endothelial cells after flow stimulation are imaged at the single‐cell level for topographical analysis. Finally, the possibility of adapting the permeability and topographical analysis using SPM for the intestinal vascular system is further evaluated. It is believed that this study will pave the way for an in situ permeability assay and structural analysis of MPS using SPM.