The Role of Arabidopsis Rubisco Activase in Jasmonate-Induced Leaf Senescence
The Role of Arabidopsis Rubisco Activase in Jasmonate-Induced Leaf Senescence
复制标题
拟南芥 Rubisco 激活酶在茉莉酸诱导的叶片衰老中的作用
DOI：
10.1104/pp.110.166595
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发表时间：
2011-02-01
期刊：
影响因子：
7.4
通讯作者：
Xie, Daoxin
中科院分区：
文献类型：
--
作者：
Shan, Xiaoyi;Wang, Junxia;Xie, Daoxin
叶片衰老是叶片发育的最后阶段，受多种发育和环境因素的调控。茉莉酸酯（JAs）已被证明可以诱导几种植物的叶片衰老，然而，茉莉酸酯诱导叶片衰老的分子机制仍不清楚。本研究采用蛋白质组学、遗传学和生理学方法，揭示了茉莉酸诱导拟南芥叶片衰老的分子基础。我们确定了35冠状蛋白不敏感1（COI1）依赖JA调节蛋白，利用二维差异凝胶电泳在拟南芥。在这35个蛋白中，Rubisco活化酶（RCA）是COI1依赖的JA抑制蛋白。我们发现，RCA的转录和蛋白质丰度水平下调JA在COI1依赖的方式。我们进一步发现，RCA的损失导致了典型的衰老相关的功能和COI1依赖的JA抑制RCA在JA诱导的叶片衰老中发挥了重要作用。
Leaf senescence, as the last stage of leaf development, is regulated by diverse developmental and environmental factors. Jasmonates (JAs) have been shown to induce leaf senescence in several plant species; however, the molecular mechanism for JA-induced leaf senescence remains unknown. In this study, proteomic, genetic, and physiological approaches were used to reveal the molecular basis of JA-induced leaf senescence in Arabidopsis (Arabidopsis thaliana). We identified 35 coronatine-insensitive 1 (COI1)-dependent JA-regulated proteins using two-dimensional difference gel electrophoresis in Arabidopsis. Among these 35 proteins, Rubisco activase (RCA) was a COI1-dependent JA-repressed protein. We found that RCA was down-regulated at the levels of transcript and protein abundance by JA in a COI1-dependent manner. We further found that loss of RCA led to typical senescence-associated features and that the COI1-dependent JA repression of RCA played an important role in JA-induced leaf senescence.