Screening and HPLC methods for carotenoids in sweetpotato, cassava and maize for plant breeding trials
Screening and HPLC methods for carotenoids in sweetpotato, cassava and maize for plant breeding trials
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DOI：
10.1016/j.foodchem.2005.10.020
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Nestel, Penelope
中科院分区：
文献类型：
--
作者：
Kimura, Mieko;Kobori, Cintia N.;Nestel, Penelope
建立了甘薯、木薯和玉米的分析方法。在橙子和鲑鱼肉甘薯，（全E）-β-胡萝卜素占主导地位，分光光度筛选和HPLC定量的结果没有显着差异。然而，在黄肉甘薯和木薯，分光光度筛选高估了HPLC值，因为存在几个次要的类胡萝卜素。除了（全E）-β-胡萝卜素，Z-异构体存在于木薯中的可观的数额。对于两种作物，用丙酮或四氢呋喃：甲醇提取。使用研钵和研杵或Polytron均化器（1：1），得到等同的结果。干玉米在室温下再水化30分钟或在85 ° C下再水化5、10或15分钟得到相同的结果。用C18和C30柱获得的浓度对于全E构型的玉米黄质、叶黄素、β-隐黄质和β-胡萝卜素没有显著差异，但它们的Z-异构体难以在用C30柱获得的色谱图中定位。用四氢呋喃：甲醇（1：1）提取得到的玉米黄质和叶黄素的结果显著较低。(c)2005爱思唯尔有限公司保留所有权利。
Analytical methods for sweetpotato, cassava and maize were developed. In orange and salmon-fleshed sweetpotatoes, (all-E)-beta-carotene predominated and results of spectrophotometric screening and HPLC quantification did not differ significantly. In yellow-fleshed sweetpotato and cassava, however, spectrophotometric screening overestimated the HPLC values because of the presence of several minor carotenoids. Aside from (all-E)-beta-carotene, Z-isomers were present in cassava in appreciable amounts. For both crops, extraction with acetone or tetrahydrofuran: methanol. (1: 1), using a mortar and pestle or a Polytron homogenizer, gave equivalent results. Rehydration of dry maize at room temperature for 30 min or at 85 degrees C for 5, 10 or 15 min gave equivalent results. Concentrations obtained with the C18 and C30 columns did not differ significantly for zeaxanthin, lutein, beta-cryptoxanthin and beta-carotene in the all-E-configuration, but their Z-isomers were difficult to locate in the chromatogram obtained with the C30 column. Extraction with tetrahydrofuran:methanol (1:1) gave significantly lower results for zeaxanthin and lutein. (c) 2005 Elsevier Ltd. All rights reserved.