Highly-Sensitive Multiplexed in vivo Imaging Using PEGylated Upconversion Nanoparticles
Highly-Sensitive Multiplexed in vivo Imaging Using PEGylated Upconversion Nanoparticles
复制标题
DOI：
10.1007/s12274-010-0036-2
复制
发表时间：
2010-10-01
期刊：
影响因子：
9.9
通讯作者：
Liu, Zhuang
中科院分区：
文献类型：
--
作者：
Cheng, Liang;Yang, Kai;Liu, Zhuang
近年来，镧系上转换纳米粒子（UCNPs）在光学成像等领域得到了广泛的应用。虽然早期的研究表明，不同镧系元素（Ln(3+)）掺杂的UCNPs表现出不同的颜色，但使用UCNPs的多色-特别是体内多路生物医学成像-很少有报道。在这项工作中，我们合成了一系列具有不同发射颜色的UCNPs，并用两亲性聚合物将其功能化以赋予其水溶性。多色体内上转换发光（UCL）成像是通过皮下注射UCNPs成像证明的，并应用于多路体内淋巴结定位。我们还使用UCNPs进行多色癌细胞标记，并通过UCL成像实现体内细胞跟踪。此外，我们首次比较了基于量子点（QD）的荧光成像和基于ucnp的UCL成像的体内成像灵敏度，发现在我们目前未优化的成像系统中，ucnp的体内检测限至少比量子点低一个数量级。我们的数据表明，由于其独特的光学特性，UCNPs在高灵敏度多路生物医学成像中具有巨大的应用潜力。
Lanthanide-based upconversion nanoparticles (UCNPs) have been widely explored in various fields, including optical imaging, in recent years. Although earlier work has shown that UCNPs with different lanthanide (Ln(3+)) dopants exhibit various colors, multicolor-especially in vivo multiplexed biomedical imaging-using UCNPs has rarely been reported. In this work, we synthesize a series of UCNPs with different emission colors and functionalize them with an amphiphilic polymer to confer water solubility. Multicolor in vivo upconversion luminescence (UCL) imaging is demonstrated by imaging subcutaneously injected UCNPs and applied in multiplexed in vivo lymph node mapping. We also use UCNPs for multicolor cancer cell labeling and realize in vivo cell tracking by UCL imaging. Moreover, for the first time we compare the in vivo imaging sensitivity of quantum dot (QD)-based fluorescence imaging and UCNP-based UCL imaging side by side, and find the in vivo detection limit of UCNPs to be at least one order of magnitude lower than that of QDs in our current non-optimized imaging system. Our data suggest that, by virtue of their unique optical properties, UCNPs have great potential for use in highly-sensitive multiplexed biomedical imaging.