CHARACTERIZATION OF A PRESYNAPTIC GLUTAMATE-RECEPTOR
CHARACTERIZATION OF A PRESYNAPTIC GLUTAMATE-RECEPTOR
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DOI：
10.1126/science.8105537
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发表时间：
1993-10-15
期刊：
影响因子：
56.9
通讯作者：
MALLET, J
中科院分区：
文献类型：
--
作者：
SMIRNOVA, T;STINNAKRE, J;MALLET, J
谷氨酸受体介导大脑中的兴奋性神经传递，对于记忆的形成和一些神经退行性疾病很重要。通过使用针对谷氨酸结合蛋白生成的抗体筛选文库，获得了编码 33 千道尔顿蛋白 (GR33) 的互补 DNA 克隆。 GR33 的序列与最近报道的突触前蛋白突触融合蛋白的序列相同。当GR33在爪蟾卵母细胞中表达时，它形成谷氨酸激活的离子通道，其药理学上与N-甲基-D-天冬氨酸受体相似，但具有不同的电生理特性。 GR33 单个假定跨膜片段中亮氨酸 278 残基的突变会影响通道的特性。因此，体内GR33可能是突触前谷氨酸受体。
Glutamate receptors mediate excitatory neurotransmission in the brain and are important in the formation of memory and in some neurodegenerative disorders. A complementary DNA clone that encoded a 33-kilodalton protein (GR33) was obtained by screening a library with an antibody generated against glutamate binding proteins. The sequence of GR33 is identical to that of the recently reported presynaptic protein syntaxin. When GR33 was expressed in Xenopus oocytes, it formed glutamate-activated ion channels that are pharmacologically similar to those of N-methyl-D-aspartate receptors but with different electrophysiological properties. Mutation of the leucine 278 residue in the single putative transmembrane segment of GR33 affects the properties of the channel. Thus, in vivo GR33 may be a presynaptic glutamate receptor.