Monitoring serotonin signaling on a subsecond time scale.
Monitoring serotonin signaling on a subsecond time scale.
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DOI：
10.3389/fnint.2013.00044
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发表时间：
2013
影响因子：
3.5
通讯作者：
Wightman RM
中科院分区：
文献类型：
--
作者：
Dankoski EC;Wightman RM
血清素调节整个大脑的各种过程，但它可能是最有名的参与抑郁症的病因和治疗。微透析研究提供了一个清晰的画面，环境中的血清素水平波动方面的行为状态和药理学操作，解剖和电生理学研究描述的位置和活动的血清素及其目标。然而，很少有联合收割机结合时间分辨率，空间精度，和化学选择性，直接评估5-羟色胺的释放和摄取。快速扫描循环伏安法（FSCV）是一种电化学方法，可以检测神经递质浓度在与突触外神经传递相同的时间和空间维度上的微小变化。亚秒测量在体内和脑切片准备使我们能够梳理除了释放和吸收的过程。这些研究特别强调了调节机制对5-羟色胺系统正常运作的重要性。本文将回顾FSCV研究的结果，并讨论这项技术的潜力，在未来的研究中的5-羟色胺系统。
Serotonin modulates a variety of processes throughout the brain, but it is perhaps best known for its involvement in the etiology and treatment of depressive disorders. Microdialysis studies have provided a clear picture of how ambient serotonin levels fluctuate with regard to behavioral states and pharmacological manipulation, and anatomical and electrophysiological studies describe the location and activity of serotonin and its targets. However, few techniques combine the temporal resolution, spatial precision, and chemical selectivity to directly evaluate serotonin release and uptake. Fast-scan cyclic voltammetry (FSCV) is an electrochemical method that can detect minute changes in neurotransmitter concentration on the same temporal and spatial dimensions as extrasynaptic neurotransmission. Subsecond measurements both in vivo and in brain slice preparations enable us to tease apart the processes of release and uptake. These studies have particularly highlighted the significance of regulatory mechanisms to proper functioning of the serotonin system. This article will review the findings of FSCV investigations of serotonergic neurotransmission and discuss this technique's potential in future studies of the serotonin system.