Mechanisms of Chinese Medicine Xinmailong's protection against heart failure in pressure-overloaded mice and cultured cardiomyocytes.
Mechanisms of Chinese Medicine Xinmailong's protection against heart failure in pressure-overloaded mice and cultured cardiomyocytes.
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中药心脉隆对压力超负荷小鼠心力衰竭的保护机制及培养心肌细胞
DOI：
10.1038/srep42843
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发表时间：
2017-02-16
影响因子：
4.6
通讯作者：
Zhang M
中科院分区：
文献类型：
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作者：
Qi J;Yu J;Tan Y;Chen R;Xu W;Chen Y;Lu J;Liu Q;Wu J;Gu W;Zhang M
心力衰竭(HF)患者死亡率高，活动能力强。心脉龙注射液是一种临床有效的治疗心力衰竭的中药。然而，XML对HF的有效机制尚不清楚，因此，这是本研究的目标。我们建立了压力超负荷性心衰小鼠模型，并比较了4个研究组：假手术组(n = 10)、横动脉缩窄组(n = 12)、美托洛尔(美托洛尔，阳性药物治疗，n = 7)和XML(XML治疗，n = 14)。用超声心动图评价心脏结构和功能的动态变化。另外，体外培养H9C2大鼠心肌细胞，用Western印迹法检测ERK1/2、AKT、GSK3β的磷酸化和GATA4在细胞核内的蛋白表达。结果表明，在TAC后2周，XML可降低左室后壁舒张期厚度，增加射血分数和短轴缩短率，从而抑制心衰。此外，XML还可抑制ERK1/2、AKT和GSK3β的磷酸化，从而抑制GATA4在细胞核内的蛋白表达(P <； 0.001)。综上所述，我们的研究结果表明，XM通过抑制ERK1/2、AKT/GSK3GATA4和β信号通路来抑制TAC诱导的心衰。
Patients with heart failure (HF) have high mortality and mobility. Xinmailong (XML) injection, a Chinese Medicine, is clinically effective in treating HF. However, the mechanism of XML’s effectiveness on HF was unclear, and thus, was the target of the present study. We created a mouse model of pressure-overload-induced HF with transverse aortic constriction (TAC) surgery and compared among 4 study groups: SHAM (n = 10), TAC (n = 12), MET (metoprolol, positive drug treatment, n = 7) and XML (XML treatment, n = 14). Dynamic changes in cardiac structure and function were evaluated with echocardiography in vivo. In addition, H9C2 rat cardiomyocytes were cultured in vitro and the phosphorylation of ERK1/2, AKT, GSK3β and protein expression of GATA4 in nucleus were detected with Western blot experiment. The results showed that XML reduced diastolic thickness of left ventricular posterior wall, increased ejection fraction and fraction shortening, so as to inhibit HF at 2 weeks after TAC. Moreover, XML inhibited the phosphorylation of ERK1/2, AKT and GSK3β, subsequently inhibiting protein expression of GATA4 in nucleus (P < 0.001). Together, our data demonstrated that XML inhibited the TAC-induced HF via inactivating the ERK1/2, AKT/GSK3β, and GATA4 signaling pathway.
罗布麻片通过抑制压力过载后的 AKT 和 ERK1/2 磷酸化来预防心脏肥大
DOI：
10.1155/2014/769515
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Qi J;Liu Q;Gong K;Yu J;Wang L;Guo L;Zhou M;Wu J;Zhang M
通讯作者：
Zhang M