Repair of the calvarial defect in goat model using magnesium-doped porous hydroxyapatite combined with recombinant human bone morphogenetic protein-2
Repair of the calvarial defect in goat model using magnesium-doped porous hydroxyapatite combined with recombinant human bone morphogenetic protein-2
复制标题
掺镁多孔羟基磷灰石联合重组人骨形态发生蛋白2修复山羊模型颅骨缺损
DOI：
10.3233/bme-171678
复制
发表时间：
2017-01-01
影响因子：
1
通讯作者：
Kang, Pengde
中科院分区：
文献类型：
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作者：
Deng, Liqing;Li, Donghai;Kang, Pengde
羟基磷灰石（Hydroxyapatite， HA）是一种具有代表性的骨修复生物材料，其成分与人类骨骼和牙齿相似。然而，纯HA在应用中受到一些不希望的特性的限制，如砖状和弱降解。在本研究中，我们通过在材料中掺杂镁（Mg）来修饰HA，并在体外研究其性能。此外，我们还评估了MgHA联合rhBMP-2对山羊颅骨缺损的修复效果。根据我们的研究结果，HA复合Mg使支架光滑，孔隙规则。体外研究表明，Mg可以增加钙的释放，这可能反映了Mg修饰的更快的降解特性。然后，MgHA提高细胞活力和增殖。与纯HA相比，MgHA可提高ALP、Collagen I和VEGF蛋白的表达（p < 0.5）。在体内研究中，MgHA在CT评价中表现出比HA更好的骨缺损愈合效果（p < 0.05），但不如MgHA/rhBMP-2 （p < 0.05）。组织学分析中，MgHA/rhBMP-2成骨效果最佳（p < 0.05），且MgHA组成骨效果显著优于纯HA组（p < 0.05）。此外，MgHA/rhBMP-2组在12周时胶原I和VEGF mRNA的表达也显著高于其他两组。综上所述，Mg对骨形成和血管生成有影响，MgHA/rhBMP-2增强了骨缺损修复效果。值得推荐给骨组织工程。
Hydroxyapatite (HA) is a representative bone repairing biomaterial for its similar composition to human bones and teeth. However, pure HA is limited in application for some unwanted characteristic, such as it is brickle and weakness in degradation. In this study, we modified HA by doping magnesium (Mg) to the material and studied its property in vitro. Besides, we also evaluated the calvarial defect repair effect using MgHA combined with rhBMP-2 in goat model. According to our outcomes, HA composited Mg made the scaffold smooth and the pore regular. In vitro study, Mg could increase the Ca releasing, which may reflect a faster degradation property modified by Mg. And then, MgHA improved the cell viability and proliferation. Furthermore, MgHA could increase the expression of ALP, Collagen I and VEGF protein compared with pure HA (p < 0.5, respectively). In the vivo study, MgHA showed a better bone defect healing effect in computed tomography (CT) evaluation compared with HA (p < 0.05), but it was inferior to the MgHA/rhBMP-2 (p < 0.05). Besides, in the histological analysis, MgHA/rhBMP-2 showed the most effective bone formation outcome (p < 0.05), and the MgHA group was significant better than the pure HA group on osteogenesis (p < 0.05). Furthermore, Collagen I and VEGF mRNA expression at 12 week in MgHA/rhBMP-2 group were also significat higher than other two groups. In conclusion, Mg had effects on bone formation and angiogenesis, and MgHA/rhBMP-2 had improved the bone defect repair effect. It is worthy of being recommended to bone tissue engineering.