Characterization of isoproturon biodegradation pathway in Sphingobium sp. YBL2
Characterization of isoproturon biodegradation pathway in Sphingobium sp. YBL2
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DOI：
10.1016/j.ibiod.2012.01.008
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发表时间：
2012-05
影响因子：
4.8
通讯作者：
Ji Zhang;Q. Hong;Qin-fen Li;Chao Li;Li Cao;Ji-Quan Sun;Xin Yan;Shun-Peng Li
中科院分区：
文献类型：
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作者：
Ji Zhang;Q. Hong;Qin-fen Li;Chao Li;Li Cao;Ji-Quan Sun;Xin Yan;Shun-Peng Li
本课题组在前期研究中分离到一株苯脲类除草剂降解菌Sphingobium sp. YBL 2。通过中间代谢产物的鉴定，研究了异丙隆（伊普）在YBL 2菌株中的降解途径。HPLC和/或MS/MS分析表明，菌株YBL 2产生3种中间代谢产物MDIPU（1-（4-异丙基苯基）-3-甲基脲）、DDIPU（1-（4-异丙基苯基）脲）和4-IA（4-异丙基苯胺）。所有这些中间代谢产物也可以被菌株YBL 2有效地降解。此外，在菌株YBL 2中检测到了IPU诱导的苯胺双加氧酶活性和邻苯二酚2，3-双加氧酶活性。结合以上结果，我们推测伊普在YBL 2菌株中的代谢途径包括两个连续的N-去甲基化，然后是脲侧链和芳香环的裂解。
In our previous study, a phenylurea herbicides degrading strain Sphingobium sp. YBL2 was isolated. In the present study, the degradation pathway of IPU (Isoproturon) in strain YBL2 was investigated by intermediate metabolites identification. HPLC and/or MS/MS analysis showed that, three intermediate metabolites, MDIPU (1-(4-isopropylphenyl)-3-methylurea), DDIPU (1-(4-isopropylphenyl) urea) and 4-IA (4-Isopropylaniline) was produced by strain YBL2. All of these intermediate metabolites could also be efficiently degraded by strain YBL2. In addition, IPU-induced aniline dioxygenase activity and catechol 2,3-dioxygenase activity were detected in strain YBL2. In combination of all these results, we deduced that the metabolic pathway of IPU in strain YBL2 involves two successive N-demethylations, followed by cleavage of the urea side chain and aromatic ring.