Multilayer checkpoints for microRNA authenticity during RISC assembly
Multilayer checkpoints for microRNA authenticity during RISC assembly
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DOI：
10.1038/embor.2011.128
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发表时间：
2011-09-01
期刊：
影响因子：
7.7
通讯作者：
Tomari, Yukihide
中科院分区：
文献类型：
--
作者：
Kawamata, Tomoko;Yoda, Mayuko;Tomari, Yukihide
MicroRNAs(MiRNAs)通过RNA诱导沉默复合体(RISC)发挥作用，RISC的核心含有Argavite(AGO)蛋白。RISC组装遵循两步路径：miRNA/miRNA*双链加载到AGO中，并在AGO内分离两条链。在这里，我们证明了miRNA链的5‘磷酸是双链装载到AGO中所必需的，而miRNA链的首选5’核苷酸以及种子区和3‘区中部的碱基配对状态对AGO起着加性锚定的作用。因此，在RISC组装过程中分多个步骤检查miRNA的真实性。
MicroRNAs (miRNAs) function through the RNA-induced silencing complex (RISC), which contains an Argonaute (Ago) protein at the core. RISC assembly follows a two-step pathway: miRNA/miRNA* duplex loading into Ago, and separation of the two strands within Ago. Here we show that the 5' phosphate of the miRNA strand is essential for duplex loading into Ago, whereas the preferred 5' nucleotide of the miRNA strand and the base-pairing status in the seed region and the middle of the 3' region function as additive anchors to Ago. Consequently, the miRNA authenticity is inspected at multiple steps during RISC assembly.