The iron-H2O2-iodide cytotoxic system.
The iron-H2O2-iodide cytotoxic system.
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DOI：
10.1084/jem.156.4.1262
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发表时间：
1982-10-01
期刊：
影响因子：
--
通讯作者：
Klebanoff SJ
中科院分区：
文献类型：
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作者：
Klebanoff SJ
描述了一种有效的抗微生物系统，其由硫酸亚铁（Fe 2+）、过氧化氢（H2 O2）和碘化物在pH 5.5的0.02 M乙酸钠缓冲液中组成。H_2O_2可以被H_2O_2产生系统葡萄糖+葡萄糖氧化酶所取代。该系统，不像髓过氧化物酶-H2 O2-卤化物系统，是无效的，当碘化物被溴，氯，或甲状腺素取代，并抑制EDTA，羟基自由基清除剂甘露醇和乙醇，磷酸盐和乳酸盐缓冲液在相同的浓度和pH值的乙酸缓冲液使用。然而，乙酸盐缓冲液可以用水代替。提出Fe ~（2+）与H_2O_2（芬顿试剂）生成OH X（或一种密切相关的物质），与碘化物相互作用形成一种或多种有毒物质。
A potent antimicrobial system is described which consists of ferrous sulfate (Fe2+), hydrogen peroxide (H2O2), and iodide in 0.02 M sodium acetate buffer pH 5.5. H2O2 could be replaced by the H2O2-generating system glucose + glucose oxidase. This system, unlike the myeloperoxidase-H2O2-halide system, was ineffective when iodide was replaced by bromide, chloride, or thyroxine, and was inhibited by EDTA, the hydroxyl radical scavengers mannitol and ethanol, and phosphate and lactate buffers at the same concentration and pH as the acetate buffer used. The acetate buffer, however, could be replaced by water. It is proposed that Fe2+ and H2O2 (Fenton's reagent) generate OH X (or a closely related substance), which interacts with iodide to form one or more toxic species.