MDA-MB-435 cells are derived from M14 melanoma cells - a loss for breast cancer, but a boon for melanoma research
MDA-MB-435 cells are derived from M14 melanoma cells - a loss for breast cancer, but a boon for melanoma research
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DOI：
10.1007/s10549-006-9392-8
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发表时间：
2007-07-01
影响因子：
3.8
通讯作者：
Johnson, Michael D.
中科院分区：
文献类型：
--
作者：
Rae, James M.;Creighton, Chad J.;Johnson, Michael D.
背景：自从 Ross 等人通过 DNA 微阵列数据分析得出结论以来，细胞系 MDA-MB-435 的组织起源一直是一个争论的问题。 (2000, Nat Genet 24(3): 227 - 235)表明它们可能是黑素细胞来源，因为它们与黑素瘤细胞系相似。我们之前已经表明，在多个实验室中保存的 MDA-MB435 细胞与 Ross 等人使用的细胞具有共同起源。并得出结论：MDA-MB-435 细胞不是乳腺癌的代表性模型。然而，我们无法确定MDA-MB-435细胞系的黑色素瘤样特性是错误分类的结果还是由于转分化为黑色素瘤样表型。方法：我们使用核型、比较基因组杂交（CGH）和微卫星多态性分析，结合基因表达和单核苷酸多态性（SNP）数据的生物信息学分析，来检验MDA-MB-435细胞系的黑色素瘤样特性是否是错误分类的结果或由于转分化为黑色素瘤样表型而导致。 MB435 细胞系源自黑色素瘤细胞系 M14。结果：我们表明 MDA-MB-435 和 M14 细胞系在细胞遗传学特征以及基因表达模式方面基本相同，并且发现的微小差异可以通过表型和基因型克隆漂移来解释。结论：所有目前可用的 MDAMB435 细胞库存均源自 M14 黑色素瘤细胞系，不能再被视为模型乳腺癌。这些细胞仍然是研究癌症转移的一个有价值的系统，自 1982 年以来使用这些细胞的大量文献为黑色素瘤研究界提供了宝贵的新资源。
Background: The tissue of origin of the cell line MDA- MB- 435 has been a matter of debate since analysis of DNA microarray data led Ross et al. ( 2000, Nat Genet 24( 3): 227 - 235) to suggest they might be of melanocyte origin due to their similarity to melanoma cell lines. We have previously shown that MDA- MB435 cells maintained in multiple laboratories are of common origin to those used by Ross et al. and concluded that MDA- MB- 435 cells are not a representative model for breast cancer. We could not determine, however, whether the melanoma- like properties of the MDA- MB- 435 cell line are the result of misclassification or due to transdifferention to a melanoma- like phenotype.Methods: We used karyotype, comparative genomic hybridization ( CGH), and microsatalite polymorphism analyses, combined with bioinformatics analysis of gene expression and single nucleotide polymorphism ( SNP) data, to test the hypothesis that the MDA- MB435 cell line is derived from the melanoma cell line M14.Results: We show that the MDA- MB- 435 and M14 cell lines are essentially identical with respect to cytogenetic characteristics as well as gene expression patterns and that the minor differences found can be explained by phenotypic and genotypic clonal drift.Conclusions: All currently available stocks of MDAMB435 cells are derived from the M14 melanoma cell line and can no longer be considered a model of breast cancer. These cells are still a valuable system for the study of cancer metastasis and the extensive literature using these cells since 1982 represent a valuable new resource for the melanoma research community.