Translational Development of a Silk-Elastinlike Protein Polymer Embolic for Transcatheter Arterial Embolization.
Translational Development of a Silk-Elastinlike Protein Polymer Embolic for Transcatheter Arterial Embolization.
复制标题
DOI：
10.1002/mabi.202100401
复制
发表时间：
2022-03
影响因子：
4.6
通讯作者：
Ghandehari, Hamidreza
中科院分区：
文献类型：
--
作者：
Hatlevik, Oyvind;Jensen, Martin;Steinhauff, Douglas;Wei, Xiaomei;Huo, Eugene;Jedrzkiewicz, Jolanta;Cappello, Joseph;Cheney, Darwin;Ghandehari, Hamidreza
用栓塞剂局部阻断肿瘤血流量是肝细胞癌经导管动脉栓塞术的关键。目前的微粒制剂不能深入渗透靶组织，并且与非常有限的治疗剂相容。丝弹性蛋白聚合物(SELP)将弹性蛋白的溶解性和丝蛋白的强度结合在一起，创建了一种易于注射的液体栓塞剂，该栓塞剂可以转变为固体仓库，适合装载药物、基因治疗剂或生物制剂。SELP作为液体溶液注射，在转化为固体水凝胶之前穿透血管系统。这篇论文的目的是在猪模型中评估自体血管栓塞术的稳定性和在7、30和90天存活间隔的生物相容性。SELP栓塞剂选择性地阻断肾脏和肝脏的血液流动，不会发生靶外梗塞。根据血管造影术的评估，在90天的研究期间，SELP肾动脉栓塞术的持久性降低。与Embball®相比，肾脏环境中微观SELP栓子的存在有所增加。与Embball®相比，肾脏和肝脏植入物中对SELP的组织学评分的炎症反应都有所减少。综上所述，可生物吸收的SELP液体栓塞剂可在体内深度穿透靶组织并选择性地栓塞血管。将液态丝状弹性蛋白聚合物(SELP)栓塞剂与临床微球Emblobe®进行比较，以用于猪肾脏和肝脏的栓塞术。与Embball®相比，SELP能够进行类似或改进的栓塞术。与Embball®相比，液体SELP栓塞剂产生的炎症反应较少，在90天的研究期间没有长期的健康问题。
Locally blocking blood flow to tumors with embolic materials is key to transcatheter arterial embolization for treating hepatocellular carcinoma. Current microparticle agents do not deeply penetrate target tissues and are compatible with a very limited selection of therapeutic agents. Silk-elastinlike protein polymers (SELPs) combine the solubility of elastin and the strength of silk to create an easily injected liquid embolic that transitions into a solid depot amenable to loading with drugs, gene therapy agents, or biologics. SELP, injected as liquid solution, penetrates the vasculature before transitioning to a solid hydrogel. The objective of this manuscript was to evaluate SELP embolization, stability, and biocompatibility at 7-, 30-, and 90-day survival intervals in a porcine model. SELP embolics selectively block blood flow in the kidneys and livers, with no off-target infarctions. As assessed with angiography, SELP renal embolization exhibited decreasing persistence for the duration of the 90-day study period. There was an increased presence of microscopic SELP emboli in the renal setting, compared to Embosphere®. Histologically scored inflammatory reactions to SELP are decreased in both the renal and hepatic implantations compared to Embosphere®. In conclusion, a bioresorbable SELP liquid embolic system deeply penetrates target tissue and selectively embolizes blood vessels in vivo. A liquid silk-elastinlike protein-based polymer (SELP) embolic is compared to a clinical microsphere, Embosphere®, for embolic applications in porcine renal and hepatic settings. SELP is capable of comparable or improved embolization with regards to Embosphere®. The liquid SELP embolic produces reduced inflammatory responses compared to Embosphere®, with no long-term health concerns over the 90-day study period.
登录
查看更多内容
影响因子：
2.9
作者：
Dreher, Matthew R.;Sharma, Karun V.;Woods, David L.;Reddy, Goutham;Tang, Yiqing;Pritchard, William F.;Chiesa, Oscar A.;Karanian, John W.;Esparza, Juan A.;Donahue, Danielle;Levy, Elliot B.;Willis, Sean L.;Lewis, Andrew L.;Wood, Bradford J.
通讯作者：
Wood, Bradford J.
影响因子：
3.7
作者：
Hatefi, A.;Cappello, J.;Ghandehari, H.
通讯作者：
Ghandehari, H.
影响因子：
4.9
作者：
Poursaid, Azadeh;Jensen, Mark Martin;Ghandehari, Hamidreza
通讯作者：
Ghandehari, Hamidreza
影响因子：
4.9
作者：
Cresce, Arthur von Wald;Dandu, Ramesh;Ghandehari, Hamidreza
通讯作者：
Ghandehari, Hamidreza
影响因子：
3.8
作者：
Miksad, Rebecca A.;Ogasawara, Sadahisa;Piscaglia, Fabio
通讯作者：
Piscaglia, Fabio