Oxidative variables, in the rat brain after sepsis induced by cecal ligation and perforation
Oxidative variables, in the rat brain after sepsis induced by cecal ligation and perforation
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DOI：
10.1097/01.ccm.0000201880.50116.12
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发表时间：
2006-03-01
影响因子：
8.8
通讯作者：
Dal-Pizzol, F
中科院分区：
文献类型：
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作者：
Barichello, T;Fortunato, JJ;Dal-Pizzol, F
目的：脓毒症存活者的精神状态改变、败血症脑病和长期认知症状的潜在机制仅有部分明确。本研究旨在评估大鼠盲肠结扎和穿孔后几种脑结构中氧化应激的不同变量。设计：前瞻性动物研究。动物基础科学实验室。干预：雄性Wistar大鼠，体重250~350g，给予盲肠结扎穿孔模型(脓毒症组)和假手术组(对照组)。实验方法和主要结果：在脓毒症早期(6h后)，通过硫代巴比妥酸反应产物和蛋白质羰基含量测定，发现大鼠海马区、小脑和皮质发生氧化损伤。在脓毒症诱导后的较长时间(12-96小时)，所有被分析的结构中都没有氧化损伤的证据。除纹状体外，在脓毒症早期(6小时)，我们发现超氧化物歧化酶活性增加，而过氧化氢酶活性没有成比例的增加，从而导致超氧化物歧化酶/过氧化氢酶关系的增加。在脓毒症诱导后12-96小时，这些酶之间的平衡在所研究的结构中恢复。结论：本研究显示的短期氧化损伤可能参与了脓毒症发展过程中中枢神经系统症状的发展，甚至是感染性脑病。超氧化物歧化酶/过氧化氢酶关系的改变在时间上与中枢神经系统氧化损伤的发生或不发生有关。
Objective: The underlying mechanisms of the changes in mental status, septic encephalopathy, and long-term cognitive symptoms in sepsis survivors have only been defined in part. The present study was undertaken to assess different variables of Oxidative stress in several brain structures after cecal ligation and perforation in the rat.Design: Prospective animal study.Setting. Animal basic science laboratory.Subjects. Male Wistar rats, weighing 250-350 g.Interventions: Rats were subjected to cecal ligation and perforation (sepsis group) with saline resuscitation (at 50 mL/kg immediately and 12 hrs after cecal ligation and perforation) or sham operation (control group).Measurements and Main Results: Oxidative damage, assessed by the thiobarbituric acid reactive species and the protein carbonyl assays, occurred early (after 6 hrs) in the course of sepsis development in the hippocampus, cerebellum, and cortex. At longer times after sepsis induction (12-96 hrs), there was no evidence of oxidative damage in all analyzed structures. Except for the striatum, earlier in sepsis development (6 hrs) we demonstrated an increase in superoxide dismutase activity without a proportional increase in catalase activity with a consequent increase in the relation of superoxide dismutase/catalase. The balance between these enzymes was restored in the studied structures 12-96 hrs after sepsis induction.Conclusions: The short-term oxidative damage demonstrated here could participate in the development of central nervous system symptoms during sepsis development, or even septic encephalopathy. The alterations in the superoxide dismutase/catalase relation were temporally related to the occurrence or not of oxidative damage in the central nervous system.