VESICLE SIZING - NUMBER DISTRIBUTIONS BY DYNAMIC LIGHT-SCATTERING
VESICLE SIZING - NUMBER DISTRIBUTIONS BY DYNAMIC LIGHT-SCATTERING
复制标题
DOI：
10.1016/s0006-3495(91)82229-9
复制
发表时间：
1991-02-01
影响因子：
3.4
通讯作者：
KRYGSMAN, P
中科院分区：
文献类型：
--
作者：
HALLETT, FR;WATTON, J;KRYGSMAN, P
描述了一种优化非负最小二乘法和指数采样拟合方法的程序，用于分析囊泡/脂质体系统的水悬浮液的动态光散射（DLS）数据。这种方法利用瑞利-甘斯-德拜形状因子的涂层球和产量的数量分布，可以直接进行比较，通过冷冻断裂电子显微镜（EM）获得的分布。在100-200 nm范围内的囊泡尺寸分布的DLS和EM结果之间获得了极好的一致性。
A procedure is described which optimizes nonnegative least squares and exponential sampling fitting methods for analysis of dynamic light scattering (DLS) data from aqueous suspensions of vesicle/liposome systems. This approach utilizes a Rayleigh-Gans-Debye form factor for a coated sphere and yields number distributions which can be compared directly to distributions obtained by freeze-fracture electron microscopy (EM). Excellent agreement between the DLS and EM results are obtained for vesicle size distributions in the 100-200-nm range.