Direct detection of proteins adsorbed on synthetic materials by matrix-assisted laser desorption ionization-mass spectrometry.
Direct detection of proteins adsorbed on synthetic materials by matrix-assisted laser desorption ionization-mass spectrometry.
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通过基质辅助激光解吸电离质谱法直接检测合成材料上吸附的蛋白质。
DOI：
10.1006/abio.1999.4201
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发表时间：
1999
影响因子：
2.9
通讯作者：
H. J. Griesser
中科院分区：
文献类型：
--
作者：
P. Kingshott;H. S. St John;H. J. Griesser
生物材料在合成表面的不可逆积累（“生物污垢”）对隐形眼镜、植入式生物医学装置、生物传感器、水净化、运输和储存系统以及海洋结构等产生不利影响。研究表明，基质辅助激光解吸电离(MALDI)-质谱法可以直接、快速、方便、高灵敏度地检测隐形眼镜上吸附的蛋白质。这种新方法允许在临床使用和体外蛋白质吸附研究中提取的样品中检测生物混杂层的次要（和主要）蛋白质成分，其水平大大低于单层覆盖。检测到的生物分子的鉴定可以通过将检测到的质量峰与已知的蛋白质分子质量或与纯化合物记录的光谱进行比较或通过单独的生化分析来完成。在不同的隐形眼镜上记录的MALDI质谱包含可分配给溶菌酶和一些较小的蛋白质的峰。这种对生物污垢早期阶段的敏感表征增强了对蛋白质/材料相互作用的理解，并有助于设计控制生物吸附过程的指导策略。
The irreversible accumulation of biological material on synthetic surfaces ("biofouling") adversely affects for instance contact lenses, implantable biomedical devices, biosensors, water purification, transport and storage systems, and marine structures. It is shown here that proteins adsorbed on contact lenses can be detected directly, rapidly, and conveniently, with high sensitivity, by matrix-assisted laser desorption ionization (MALDI)-mass spectrometry. This new approach allows detection of minor (and major) proteinaceous constituents of biofouled layers on samples retrieved from clinical usage and in vitro protein adsorption studies, at levels substantially below monolayer coverage. Identification of the detected biological molecules can be done by comparison of the detected mass peaks with known protein molecular masses or with spectra recorded of pure compounds or by separate biochemical assays. The MALDI mass spectra recorded on different contact lenses contain peaks assignable to lysozyme and a number of smaller proteins. Such sensitive characterization of the early stages of biofouling enhances the understanding of protein/materials interactions and assists in designing guided strategies toward control of biological adsorption processes.
影响因子：
2.9
作者：
Huang, ZH;Shen, TL;Watson, JT
通讯作者：
Watson, JT
影响因子：
7.4
作者：
Worrall, TA;Cotter, RJ;Woods, AS
通讯作者：
Woods, AS