Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns
Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns
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DOI：
10.1128/aac.00760-08
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发表时间：
2009-02-01
影响因子：
4.9
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
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作者：
Dai, Tianhong;Tegos, George P.;Hamblin, Michael R.
一种工程化的醋酸壳聚糖绷带制剂（HemCon）被用作止血敷料，其化学结构表明它也应该是抗菌的。我们以前表明，当醋酸壳聚糖绷带应用于感染致病性生物发光细菌（铜绿假单胞菌，奇异变形杆菌和金黄色葡萄球菌）的小鼠的全层切除伤口时，它能够迅速杀死细菌并使小鼠免于致命感染。伤口愈合也受到刺激。在本研究中，我们询问了醋酸壳聚糖绷带是否可以作为局部抗菌敷料，当它被应用于三度烧伤的小鼠污染的两种细菌物种（铜绿假单胞菌和奇异假单胞菌）。初步实验确定了烧伤时间的长度和在未经治疗的小鼠中产生致命感染所需的细菌数量，并确定了醋酸壳聚糖绷带可以粘附在感染的烧伤处长达21天。在铜绿假单胞菌感染的情况下，用醋酸壳聚糖绷带治疗的小鼠的存活率为73.3%（而用纳米晶银敷料治疗的小鼠的存活率为27.3% [P = 0.0055]，未治疗的小鼠的存活率为13.3% [P < 0.0002]）。感染奇异变形杆菌的存活率分别为66.7%、62.5%和23.1%。定量生物发光信号显示，醋酸壳聚糖绷带有效地控制了烧伤中细菌的生长，并防止了全身性脓毒症的发展，如血培养所示。这些数据表明醋酸壳聚糖绷带在预防致命性烧伤感染方面是有效的。
An engineered chitosan acetate bandage preparation (HemCon) is used as a hemostatic dressing, and its chemical structure suggests that it should also be antimicrobial. We previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal infections. Wound healing was also stimulated. In the present study, we asked whether a chitosan acetate bandage could act as a topical antimicrobial dressing when it was applied to third-degree burns in mice contaminated with two of these bacterial species (P. aeruginosa and P. mirabilis). Preliminary experiments established the length of burn time and the number of bacteria needed to produce fatal infections in untreated mice and established that the chitosan acetate bandage could adhere to the infected burn for up to 21 days. In the case of P. aeruginosa infections, the survival rate of mice treated with the chitosan acetate bandage was 73.3% (whereas the survival rate of mice treated with a nanocrystalline silver dressing was 27.3% [P = 0.0055] and that of untreated mice was 13.3% [P < 0.0002]). For P. mirabilis infections, the comparable survival rates were 66.7%, 62.5%, and 23.1% respectively. Quantitative bioluminescent signals showed that the chitosan acetate bandage effectively controlled the growth of bacteria in the burn and prevented the development of systemic sepsis, as shown by blood culture. These data suggest that chitosan acetate bandage is efficacious in preventing fatal burn infections.