The biobank of the Norwegian mother and child cohort Study: A resource for the next 100 years
The biobank of the Norwegian mother and child cohort Study: A resource for the next 100 years
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DOI：
10.1007/s10654-006-9041-x
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发表时间：
2006-08-01
影响因子：
13.6
通讯作者：
Hoppin, Jane A.
中科院分区：
文献类型：
--
作者：
Ronningen, Kjersti S.;Paltiel, Liv;Hoppin, Jane A.
导言：生物材料的长期储存是任何流行病学研究的一个关键组成部分。在设计标本库时，需要努力平衡未来对样本的需求与及时处理和储存样本所需的后勤限制。目的：在挪威母亲和儿童队列研究（MoBa）中，生物库负责长期储存来自孕妇、其伴侣及其子女的380 000多份生物样本，储存时间长达100年。方法：生物标本包括全血、血浆、DNA和尿液;样本收集于挪威的50家医院。所有样本均通过普通邮件发送至奥斯陆的生物库，在那里对样本进行登记、等分和DNA提取。DNA储存在-20 ℃，而全血、尿液和血浆储存在-80 ℃。结果：截至2006年7月，已收集、处理并储存在生物库中的样本超过227 000套。目前每天收到250-300套。生物库的一个重要组成部分是质量控制程序。结论：由于生物标本和问卷调查数据的独特组合，MoBa研究将成为未来许多研究遗传和环境因素对妊娠结局以及对人类发病率、死亡率和健康的单独和综合影响的资源。
Introduction: Long-term storage of biological materials is a critical component of any epidemiological study. In designing specimen repositories, efforts need to balance future needs for samples with logistical constraints necessary to process and store samples in a timely fashion. Objectives: In the Norwegian Mother and Child Cohort Study (MoBa), the Biobank was charged with long-term storage of more than 380,000 biological samples from pregnant women, their partners and their children for up to 100 years. Methods: Biological specimens include whole blood, plasma, DNA and urine; samples are collected at 50 hospitals in Norway. All samples are sent via ordinary mail to the Biobank in Oslo where the samples are registered, aliquoted and DNA extracted. DNA is stored at -20 degrees C while whole blood, urine and plasma are stored at -80 degrees C. Results: As of July 2006, over 227,000 sample sets have been collected, processed and stored at the Biobank. Currently 250-300 sets are received daily. An important part of the Biobank is the quality control program. Conclusion: With the unique combination of biological specimens and questionnaire data, the MoBa Study will constitute a resource for many future investigations of the separate and combined effects of genetic, environmental factors on pregnancy outcome and on human morbidity, mortality and health in general.