Monodispersed Bioactive Glass Submicron Particles and Their Effect on Bone Marrow and Adipose Tissue-Derived Stem Cells
Monodispersed Bioactive Glass Submicron Particles and Their Effect on Bone Marrow and Adipose Tissue-Derived Stem Cells
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DOI：
10.1002/adhm.201300126
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发表时间：
2014-01-01
影响因子：
10
通讯作者：
Jones, Julian R.
中科院分区：
文献类型：
--
作者：
Tsigkou, Olga;Labbaf, Sheyda;Jones, Julian R.
球形单分散的生物活性颗粒是纳米复合材料合成的潜在候选物，或作为可注射颗粒，其可被细胞内化以局部持续递送无机治疗离子（例如，钙或锶）。颗粒也可能从多孔生物活性玻璃和溶胶-凝胶混合支架中释放出来，因为它们降解;因此，研究它们与细胞的相互作用至关重要。球形单分散生物活性玻璃颗粒（单SMBG），直径为215 +/- 20 nm的合成使用改进的Stober过程。共聚焦和透射电子显微镜表明，单SMBG内化的人骨髓（MSC）和脂肪来源的干细胞（ADSC），并位于细胞囊泡和细胞质中。观察到细胞内的颗粒溶解。Alcohol Blue、MTT和Cyquant测定表明，50 μ g/mL（-1）的单一SMBG不会显著抑制MSC或ADSC的代谢活性。然而，在较高浓度（100和200 g mL（-1））下，观察到代谢活性和总DNA略有下降。在无成骨补充剂的情况下，Mono-SMBG不诱导ALcase 1活性（成骨分化的早期标志物）;然而，在其存在下实现了成骨分化。此外，大量的颗粒被细胞内化，但对细胞行为几乎没有影响。
Spherical monodispersed bioactive particles are potential candidates for nanocomposite synthesis or as injectable particles that could be internalized by cells for the local sustained delivery of inorganic therapeutic ions (e.g., calcium or strontium). Particles are also likely to be released from porous bioactive glass and sol-gel hybrid scaffolds as they degrade; thus, it is vital to investigate their interaction with cells. Spherical monodispersed bioactive glass particles (mono-SMBG), with diameters of 215 +/- 20 nm are synthesized using a modified Stober process. Confocal and transmission electron microscopy demonstrate that mono-SMBGs are internalized by human bone marrow (MSCs) and adipose-derived stem cells (ADSCs) and located within cell vesicles and in the cytoplasm. Particle dissolution inside the cells is observed. Alamar Blue, MTT and Cyquant assays demonstrate that 50 g mL(-1) of mono-SMBGs did not inhibit significantly MSC or ADSC metabolic activity. However, at higher concentrations (100 and 200 g mL(-1)) small decrease in metabolic activity and total DNA is observed. Mono-SMBG did not induce ALPase activity, an early marker of osteogenic differentiation, without osteogenic supplements; however, in their presence osteogenic differentiation is achieved. Additionally, large numbers of particles are internalized by the cells but have little effect on cell behavior.