BOVINE SERUM-ALBUMIN - CHARACTERIZATION OF A FATTY-ACID BINDING-SITE ON THE N-TERMINAL PEPTIC FRAGMENT USING A NEW SPIN-LABEL
BOVINE SERUM-ALBUMIN - CHARACTERIZATION OF A FATTY-ACID BINDING-SITE ON THE N-TERMINAL PEPTIC FRAGMENT USING A NEW SPIN-LABEL
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DOI：
10.1021/bi00320a006
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
FELDHOFF, RC
中科院分区：
文献类型：
--
作者：
HSIA, JC;WONG, LT;FELDHOFF, RC
一种新的自旋标记4-(L-glutamo)-4‘’-[(1-oxy-2，2，5，5-tetramethyl-3L-pyrrolidinyl)amino]-3，3‘’-dinitrodiphenyl砜被证明与牛血清白蛋白的1个高亲和力结合部位(K=5倍)结合。104M-1，n=1)。对自旋标记与BSA的氨基末端部分(胃酸片段PB)和羧基末端部分(胃酸片段PA)及其络合物(PA-PB)的结合分析表明，自旋标记与位于PB上的长链脂肪酸结合部位结合。讨论了自旋标记的新的专一性在表征该结合部位的有用性。
A new spin-label, 4-(L-glutamo)-4''-[(1-oxy-2,2,5,5-tetramethyl-3L-pyrrolidinyl)amino]-3,3''-dinitrodiphenyl sulfone, is shown to bind to 1 high-affinity binding site on bovine serum albumin [BSA] (K = 5 .times. 104 M-1, n = 1). Analysis of the binding of the spin-label to the amino-terminal half (peptic fragment PB) and the carboxy-terminal half (peptic fragment PA) of BSA, and their complex (PA-PB), indicates that the spin-label binds to a long-chain fatty acid binding site located on PB. The usefulness of the novel specificity of the spin-label in characterizing this binding site is discussed.