Dissection of the Genetic Basis of Resistance to Stem Rot in Cultivated Peanuts (Arachis hypogaea L.) through Genome-Wide Association Study.
Dissection of the Genetic Basis of Resistance to Stem Rot in Cultivated Peanuts (Arachis hypogaea L.) through Genome-Wide Association Study.
复制标题
DOI：
10.3390/genes14071447
复制
发表时间：
2023-07-14
期刊：
影响因子：
3.5
通讯作者：
Liao, Boshou
中科院分区：
文献类型：
--
作者：
Yan, Liying;Song, Wanduo;Wang, Zhihui;Yu, Dongyang;Sudini, Hari;Kang, Yanping;Lei, Yong;Huai, Dongxin;Chen, Yuning;Wang, Xin;Wang, Qianqian;Liao, Boshou
花生（Arachishypogaea）是世界范围内重要的油籽和经济作物，为人类营养提供食用油和蛋白质的重要来源。然而，由Athelia rolfsii引起的茎腐病的发生给花生种植带来了重大挑战，造成了巨大的产量损失。在这项研究中，通过在三种环境中用受 A. rolfsii 侵染的燕麦粒接种田间植物，评估了一组 202 个花生品种对茎腐病的抵抗力。亚种种质每个环境的平均疾病指数值。 fasitiate 和 subsp。 Hypogaea 没有表现出显着差异。与其他生态区的材料相比，来自中国南方的材料表现出最低的疾病指数值。我们使用全基因组重测序来分析材料的基因型，并通过全基因组关联研究（GWAS）来识别与茎腐病抗性相关的重要 SNP。共鉴定出121个与花生抗茎腐病相关的显着SNP，表型变异解释（PVE）范围为12.23%至15.51%。共注释了23个显着SNP上下游100 kb范围内的27个候选基因，这些基因具有识别、信号转导和防御反应相关的功能。这些重要的SNP和候选基因为进一步验证和分子育种以提高花生的茎腐病抗性提供了有价值的信息。
Peanut (Arachis hypogaea) is an important oilseed and cash crop worldwide, contributing an important source of edible oil and protein for human nutrition. However, the incidence of stem rot disease caused by Athelia rolfsii poses a major challenge to peanut cultivation, resulting in significant yield losses. In this study, a panel of 202 peanut accessions was evaluated for their resistance to stem rot by inoculating plants in the field with A. rolfsii-infested oat grains in three environments. The mean disease index value of each environment for accessions in subsp. fasitigiate and subsp. hypogaea showed no significant difference. Accessions from southern China displayed the lowest disease index value compared to those from other ecological regions. We used whole-genome resequencing to analyze the genotypes of the accessions and to identify significant SNPs associated with stem rot resistance through genome-wide association study (GWAS). A total of 121 significant SNPs associated with stem rot resistance in peanut were identified, with phenotypic variation explained (PVE) ranging from 12.23% to 15.51%. A total of 27 candidate genes within 100 kb upstream and downstream of 23 significant SNPs were annotated, which have functions related to recognition, signal transduction, and defense response. These significant SNPs and candidate genes provide valuable information for further validation and molecular breeding to improve stem rot resistance in peanut.
登录
查看更多内容
影响因子：
5.4
作者：
Pang, Yunlong;Wu, Yuye;Liu, Shubing
通讯作者：
Liu, Shubing
影响因子：
4.5
作者：
Liu X;Huang M;Fan B;Buckler ES;Zhang Z
通讯作者：
Zhang Z
影响因子：
3.3
作者：
Raj A;Stephens M;Pritchard JK
通讯作者：
Pritchard JK
抗倒伏和产量的遗传分析为绿色革命后水稻育种提供了见解
影响因子：
13.8
作者：
Guo Z;Liu X;Zhang B;Yuan X;Xing Y;Liu H;Luo L;Chen G;Xiong L
通讯作者：
Xiong L
影响因子：
0.8
作者：
Branch, W. D.;Culbreath, A. K.
通讯作者：
Culbreath, A. K.