Tetrode recording of rat CA1 place cells in an observational spatial working memory task.
Tetrode recording of rat CA1 place cells in an observational spatial working memory task.
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DOI：
10.1016/j.xpro.2022.101501
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发表时间：
2022-09-16
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社会观察通过激活海马区细胞模式促进空间学习。在这里，我们描述了一个观察性空间工作记忆任务，以研究支持观察性学习的神经回路。这种方法训练观察者大鼠通过观察演示者的空间轨迹，同时记录他们的海马区CA1区细胞在几个小时的过程中的活动，来学习运行T形迷宫。该协议提供了一种工具，可以在社会环境中研究人群水平的神经活动。有关本议定书的使用和执行的完整细节，请参阅。构建用于体内细胞外记录的四极管和超驱动装置用于观察性工作记忆任务的行为训练和测试立体定位法植入超驱动装置并在自由活动的大鼠中进行记录出版者注：进行任何实验方案都需要遵守当地关于实验室安全和伦理的机构指南。社会观察通过激活海马区细胞模式促进空间学习。在这里，我们描述了一个观察性空间工作记忆任务，以研究支持观察性学习的神经回路。这种方法训练观察者大鼠通过观察演示者的空间轨迹，同时记录他们的海马区CA1区细胞在几个小时的过程中的活动，来学习运行T形迷宫。该协议提供了一种工具，可以在社会环境中研究人群水平的神经活动。
Social observation facilitates spatial learning by activation of hippocampal place cell patterns. Here, we describe an observational spatial working memory task to investigate the neural circuits underlying observational learning. This approach trains observer rats to learn to run a T-maze by observing a demonstrator’s spatial trajectory while recording their hippocampal CA1 place cell activities in a course of several hours. The protocol provides a tool to study neural activities at population level in a social setting. For complete details on the use and execution of this protocol, please refer to. Construction of tetrodes and hyperdrive for in vivo extracellular recording Behavioral training and test of an observational working memory task Stereotaxic implantation of hyperdrive and recording in freely moving rats Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Social observation facilitates spatial learning by activation of hippocampal place cell patterns. Here, we describe an observational spatial working memory task to investigate the neural circuits underlying observational learning. This approach trains observer rats to learn to run a T-maze by observing a demonstrator’s spatial trajectory while recording their hippocampal CA1 place cell activities in a course of several hours. The protocol provides a tool to study neural activities at population level in a social setting.