Bifidobacterium longum as a delivery system for cancer gene therapy:: Selective localization and growth in hypoxic tumors
Bifidobacterium longum as a delivery system for cancer gene therapy:: Selective localization and growth in hypoxic tumors
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DOI：
10.1038/sj.cgt.7700122
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发表时间：
2000-02-01
影响因子：
6.4
通讯作者：
Taniguchi, S
中科院分区：
文献类型：
--
作者：
Yazawa, K;Fujimori, M;Taniguchi, S
癌症基因治疗的一个根本障碍是将抗癌基因产物特异性递送至实体瘤，然而目前不存在专门针对实体瘤的系统递送系统。一种家养细菌，长双歧杆菌，是非致病性和厌氧的，选择性地定位并在多种类型中增殖。全身应用后的小鼠实体瘤。在这份报告中，我们进一步描述了一种癌症基因治疗的新方法，其中基因工程双歧杆菌被用作肿瘤特异性载体。与野生型长双歧杆菌类似，基因工程改造的长双歧杆菌只能在肿瘤组织中检测到，而在静脉注射后对正常小鼠组织的大规模调查中并未发现。这一发现强烈表明，双歧杆菌等专性厌氧细菌可以用作癌症基因治疗的高度特异性基因递送载体。
A fundamental obstacle in gene therapy for cancer is the specific delivery of an anticancer gene product to a solid tumor, and yet no systemic delivery system that specifically targets solid tumors currently exists. A strain of domestic bacteria, Bifidobacterium longum, which is nonpathogenic and anaerobic, selectively localized and proliferated in several types of. mouse solid tumors after systemic application. In this report, we further describe a novel approach to cancer gene therapy in which genetically engineered Bifidobacterium is used as a tumor-specific vector. Similarly to wild-type B. longum, genetically engineered B. longum could be detected in tumor tissue only and was not found in a large survey of normal mouse tissues after intravenous injection. This finding strongly suggests that obligate anaerobic bacteria such as Bifidobacterium can be used as highly specific gene delivery vectors for cancer gene therapy.