Cationic Amphiphilic Polymers with Antimicrobial Activity for Oral Care Applications: Eradication of S. mutans Biofilm.
Cationic Amphiphilic Polymers with Antimicrobial Activity for Oral Care Applications: Eradication of S. mutans Biofilm.
复制标题
具有抗菌活性的阳离子两亲性聚合物，用于口腔护理应用：消除Mutans生物膜。
DOI：
10.1021/acs.biomac.6b01598
复制
发表时间：
2017-01-09
影响因子：
6.2
通讯作者：
Kuroda K
中科院分区：
文献类型：
--
作者：
Takahashi H;Nadres ET;Kuroda K
研究了阳离子两亲性甲基丙烯酸酯聚合物对致龋菌S.研究了变形杆菌。采用可逆加成-断裂链转移聚合法合成了阳离子均聚物PE 0和含31mol%甲基丙烯酸乙酯的共聚物PE 31。这些聚合物对沙门氏菌具有杀菌活性。变形杆菌，并通过杀死嗜酸性细菌来防止生物膜的形成。在1000 μg/mL的浓度下，当孵育2小时时，聚合物减少超过80%的生物膜生物量。当使用移液管将具有生物膜的聚合物测定溶液剧烈混合30秒时，在250 μg/mL的聚合物浓度下除去超过50%的生物膜质量。氯己定和阳离子表面活性剂在相同浓度下未能减少生物膜质量。PE 0在去除生物膜方面是最有效的，并且当孵育10分钟时，对人牙龈成纤维细胞和牙周膜干细胞没有显示出任何显著的细胞毒性。
The anti-bacterial and anti-biofilm activities of cationic amphiphilic methacrylate polymers against cariogenic bacterium S. mutans were investigated. Cationic homopolymer PE0 and copolymer PE31 containing 31 mole% of ethyl methacrylate were synthesized by reversible addition-fragmentation chain transfer polymerization. These polymers displayed bactericidal activity towards S. mutans and prevented biofilm formation by killing the planktonic bacteria. At a concentration of 1000 μg/mL when incubated for 2 hours the polymers reduced more than 80% of biofilm biomass. When the polymer assay solution with the biofilm was vigorously mixed using a pipette for 30 seconds, more than 50% of biofilm mass was removed at a polymer concentration of 250 μg/mL. Chlorhexidine and a cationic surfactant failed to reduce the biofilm mass at the same concentration. PE0 was the most effective in removing biofilm and did not show any significant cytotoxicity to human gingival fibroblast and periodontal ligament stem cells when incubated for 10 minutes.