Different concentrations of ascorbyl-2-monophosphate-magnesium as dietary sources of vitamin C for seabass, Lates calcarifer
Different concentrations of ascorbyl-2-monophosphate-magnesium as dietary sources of vitamin C for seabass, Lates calcarifer
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DOI：
10.1016/s0044-8486(96)01489-5
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发表时间：
1997-05
期刊：
影响因子：
4.5
通讯作者：
W. Phromkunthong;M. Boonyaratpalin;V. Storch
中科院分区：
文献类型：
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作者：
W. Phromkunthong;M. Boonyaratpalin;V. Storch
在本研究中，在鲈鱼中测试了不同浓度的抗坏血酸-2-单磷酸镁（APM；0、30、60 或 100 mg kg−1 饮食）的效果，为期 6 周。饲喂未补充维生素 C 的饲料的鱼表现出各种临床症状，如鳍糜烂、游动行为不稳定和脊柱畸形。在喂食缺乏 APM 的饲料的鱼的鳃和肾脏中发现了一些病理变化。鳃最显着的特征是外上皮细胞层变形、次级板层上皮组织分离和呼吸道上皮增生。还观察到红细胞在次级板层上皮细胞外层和内层之间的空间中积聚。喂食不含 APM 饲料的鱼的肾脏发生了变化；这些变化包括线粒体的肿胀、变形和融合，以及内质网的膜轮。喂食含有 APM 的饲料的鲈鱼的鳃或肾脏近端小管细胞没有表现出任何这些异常。
In this study, the effects of different concentrations of ascorbyl-2-monophosphate-magnesium (APM; 0, 30, 60, or 100 mg kg−1diet) were tested in seabass for 6 weeks. Fish fed the diet without vitamin C supplementation showed various clinical signs such as fin erosion, erratic swimming behavior and spinal deformities. Several pathological changes were found in the gills and kidneys of fish fed a diet deficient in APM. The most remarkable feature in the gills was deformation of the outer epithelial cell layer, separation of the epithelial tissues of the secondary lamellae, and hyperplasia of the respiratory epithelium. An accumulation of red blood cells in the space between the outer and inner layer of epithelial cells of secondary lamellae was also observed. Changes in the kidneys were found in fish fed the APM-free diet; these changes included swelling, deformation, and fusion of mitochondria, as well as membrane whorls of endoplasmic reticulum. Seabass fed APM-containing diets did not show any of these abnormalities in the gills or proximal tubule cells of the kidney.