How to optimize the drop plate method for enumerating bacteria
How to optimize the drop plate method for enumerating bacteria
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DOI：
10.1016/s0167-7012(00)00241-4
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发表时间：
2001-03-01
影响因子：
2.2
通讯作者：
Heersink, J
中科院分区：
文献类型：
--
作者：
Herigstad, B;Hamilton, M;Heersink, J
滴板（DP）法可用于测定已知烧杯体积中的活悬浮细菌数量。滴板法比扩散板法（SP）具有一些优点。将液滴分配到琼脂平板上所需的时间和精力比将等量的总样品体积分散到琼脂中所需的时间和精力要少。把样本一滴一滴地分散。菌落计数可以更快地进行，并且可能更准确。尽管滴板法在实验室中已经存在多年，但它尚未标准化。一些技术人员使用10倍稀释。其他人使用两个。一些技术人员的板总体积为0.1毫升，其他板0.2毫升。这些因素的最佳组合将是有用的，以了解当进行滴板法。这项调查进行，以确定（i）细菌密度估计的标准偏差，（ii）进行滴板程序的成本，（iii）最佳滴板设计，（iv）滴板法相比的优势：标准涂布板法。最优设计是在固定成本下实现最小标准差的因子设置的组合。计算机模拟技术和回归分析被用来表示作为烧杯体积，稀释因子的函数的标准偏差。和体积铺板。标准偏差表达式也适用于扩散板法。(C)出版社：Elsevier Science B.V.
The drop plate (DP) method can be used to determine the number of viable suspended bacteria in a known beaker volume. The drop plate method has some advantages over the spread plate (SP) method. Less time and effort are required to dispense the drops onto an agar plate than to spread an equivalent total sample volume into the agar. By distributing the sample in drops. colony counting can be done faster and perhaps more accurately. Even though it has been present in the laboratory for many years, the drop plate method has not been standardized. Some technicians use 10-fold dilutions. others use twofold. Some technicians plate a total volume of 0.1 mi, others plate 0.2 mi. The optimal combination of such factors would be useful to know when performing the drop plate method.This investigation was conducted to determine (i) the standard deviation of the bacterial density estimate, (ii) the cost of performing the drop plate procedure, (iii) the optimal drop plate design, and (iv) the advantages of the drop plate method in comparison to the: standard spread plate method. The optimal design is the combination of factor settings that achieves the smallest standard deviation for a fixed cost. Computer simulation techniques and regression analysis were used to express the standard deviation as a function of the beaker volume, dilution factor. and volume plated. The standard deviation expression is also applicable to the spread plate method. (C) 2001 Published by Elsevier Science B.V.