Endorphinergic modulation of immune function: potent action of the dipeptide glycyl-L-glutamine.
Endorphinergic modulation of immune function: potent action of the dipeptide glycyl-L-glutamine.
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DOI：
10.1016/0024-3205(87)90490-5
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发表时间：
1987-07
期刊：
影响因子：
6.1
通讯作者：
H. W. McCain;J. Bilotta;I. Lamster
中科院分区：
文献类型：
--
作者：
H. W. McCain;J. Bilotta;I. Lamster
GLG （glyyl - l- glutamine）是b -内啡肽的羧基末端二肽，可抑制脑干神经元的活性。它也与b -内啡肽一起出现在垂体分泌囊泡中，表明该二肽具有神经分泌作用。我们利用植物血凝素（PHA）胚发生和豆豆蛋白A （ConA）抑制细胞诱导试验评估了该化合物的潜在免疫调节作用。然而，低剂量（10 ~ 12M）的GLG增强了人淋巴细胞对PHA诱导的囊胚发生的反应；高剂量二肽（10−7M）持续观察到免疫抑制。在抑制细胞诱导实验中，当GLG与ConA一起使用时，我们观察到抑制活性的剂量依赖性抑制。这些结果清楚地表明，GLG对至少两个免疫功能指标产生剂量依赖性的双向调节，并证实存在第二垂体肽，具有潜在的有效免疫调节作用。
Glycyl-L-glutamine (GLG), the carboxy terminal dipeptide of B-endorphin, inhibits brainstem neuronal activity. It also occurs along with B-endorphin in pituitary secretory vesicles suggesting a neurosecretory role for this dipeptide. We have evaluated potential immunoregulatory actions of this compound using the Phytohemaglutinin (PHA) blastogenesis and the concanavalin A (ConA) suppressor cell induction assays. GLG in low doses (10−12M) enhanced the response of human lymphocytes to PHA induced blastogenesis, however; with higher doses of the dipeptide (10−7M) immunosuppression was consistently observed. In the suppressor cell induction assay, when GLG was used together with ConA, we observed a dose-dependent inhibition of suppressor activity. These results clearly indicate that GLG produces a dose dependent bidirectional modulation of at least two indicies of immune function, and confirm the presence of a second pituitary peptide with the potential for potent immunomodulatory action.