Isolation and Identification of Phenolic Compounds Accumulated in Brown Rice Grains Ripened under High Air Temperature
Isolation and Identification of Phenolic Compounds Accumulated in Brown Rice Grains Ripened under High Air Temperature
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DOI：
10.1021/jf403416e
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发表时间：
2013-12-11
影响因子：
6.1
通讯作者：
Kondo, Motohiko
中科院分区：
文献类型：
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作者：
Nakano, Hiroshi;Ono, Hiroshi;Kondo, Motohiko
本研究旨在利用耐热品种“房乙女”、不耐热品种“初星”和中间品种“越光”，调查在高气温下成熟的糙米粒中化合物浓度在成熟过程中增加或减少的情况。 6-O-阿魏酰蔗糖 (1)、3',6-二-O-芥子酰蔗糖 (2)、3'-O-芥子酰-6-O-阿魏酰蔗糖 (3)、3',6-二-O-阿魏酰蔗糖 (4)、环阿魏酸 (5) 和 24-亚甲基环阿魏酸 (6)糙米粒的提取物。在光谱分析的基础上阐明了分离的化合物(1-6)的结构。高气温下成熟的籽粒中化合物2、3和6的平均浓度显着高于常温下成熟的籽粒。相比之下，在高气温下成熟的籽粒中化合物5的平均浓度明显低于在常温下成熟的籽粒。因此，化合物 2、3、5 和 6 构成所用品种热应激的潜在生物标志物。 Fusaotome 籽粒中化合物 4 的平均浓度是所有品种中最高的。相比之下，Fusaotome 籽粒中化合物 5 的平均浓度最低。因此，耐热Fusaotome的独特成分结合了高浓度的化合物4和低浓度的化合物5。
This study aimed to examine the compounds increasing or decreasing in concentration in brown rice grains ripened under high air temperature during ripening using a heat-tolerant cultivar Fusaotome, a heat-intolerant cultivar Hatsuboshi, and an intermediate cultivar Koshihikari. 6-O-Feruloylsucrose (1), 3',6-di-O-sinapoylsucrose (2), 3'-O-sinapoyl-6-O-feruloylsucrose (3), 3',6-di-O-feruloylsucrose (4), cycloartenyl ferulate (5), and 24-methylenecycloartanyl ferulate (6) were isolated from the extracts of brown rice grains. The structures of the isolated compounds (1-6) were elucidated on the basis of spectroscopic analyses. The mean concentrations of compounds 2, 3, and 6 in the grains ripened under high air temperature were markedly higher than those ripened under normal air temperature. In contrast, the mean concentration of compound 5 in the grains ripened under high air temperature was markedly lower than those ripened under normal air temperature. Thus, compounds 2, 3, 5, and 6 constitute potential biomarkers of heat stress in the cultivars used. The mean concentrations of compound 4 in the grains of Fusaotome were the highest in all cultivars. In contrast, the mean concentration of compound 5 in the grains of Fusaotome was the lowest. Therefore, the unique composition of heat-tolerant Fusaotome combines a high concentration of compound 4 with a low concentration of compound 5.