A SEROTONIN-CONTAINING PATHWAY FROM THE AREA POSTREMA TO THE PARABRACHIAL NUCLEUS IN THE RAT
A SEROTONIN-CONTAINING PATHWAY FROM THE AREA POSTREMA TO THE PARABRACHIAL NUCLEUS IN THE RAT
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DOI：
10.1016/0306-4522(85)90281-7
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发表时间：
1985-01-01
期刊：
影响因子：
3.3
通讯作者：
VANDERKOOY, D
中科院分区：
文献类型：
--
作者：
LANCA, AJ;VANDERKOOY, D
应用逆行追踪-免疫荧光技术，观察了大鼠臂旁核投射细胞群与最后区5-羟色胺（5-HT）免疫反应阳性细胞群的关系。在臂旁区注射逆行荧光示踪剂True Blue，3天后对动物进行灌注。连续低温恒温切片进行5-HT免疫荧光处理。在最后区发现三组不同的标记细胞。观察到投射至臂旁核的True Blue阳性细胞（高达250个/切片）分布在整个最后区。在帕吉林（一种单胺氧化酶抑制剂）处理的动物中，观察到大量的多巴胺能细胞（高达125个/切片）分布在整个最后区。有一种倾向，较重的5-HT免疫反应细胞分布在背侧三分之二的最后区。 还观察到双标记细胞。在投射到臂旁核的真蓝标记细胞中，20%为5-HT免疫反应阳性。在5-HT免疫反应性人群中，39%被真蓝逆行标记。因此，一个新的神经元通路从最后区的臂旁核的描述，这条通路可能是重要的上行传输和调制的化学和内脏感觉输入。
A combined retrograde tracing-immunofluorescent technique was used to identify the relationships between the cellular population projecting to the parabrachial nucleus and the serotonin[5-hydroxytryptamine, 5-HT]-immunoreactive cell population of the area postrema in rats. The retrograde fluorescent tracer True Blue was injected in the parabrachial region and 3 days later the animals were perfused. Serial cryostat sections were processed for 5-HT immunofluorescence. Three different groups of labeled cells were identified in the area postrema. True Blue-positive cells (up to 250/section) that project to the parabrachial nucleus were observed distributed throughout the area postrema. In the pargyline (a monoamine oxidase inhibitor)-treated animals a large number of serotonergic cells (up to 125/section) was observed distributed throughout the area postrema. There was a tendency to a heavier distribution of 5-HT immunoreactive cells in the dorsal two-thirds of the area postrema. Double-labeled cells were also seen. Of the True Blue-labeled cells projecting to the parabrachial nucleus 20% were 5-HT-immunoreactive. Of the 5-HT-immunoreactive population 39% were retrogradely labeled with True Blue. Thus a new serotonergic pathway from the area postrema to the parabrachial nucleus is described; this pathway may be important in the ascending transmission and modulation of chemical and visceral sensory input.