Whole-genome reciprocal BLAST analysis reveals that Planctomycetes do not share an unusually large number of genes with Eukarya and Archaea
Whole-genome reciprocal BLAST analysis reveals that Planctomycetes do not share an unusually large number of genes with Eukarya and Archaea
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DOI：
10.1128/aem.00429-06
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发表时间：
2006-10-01
影响因子：
4.4
通讯作者：
Rocap, Gabrielle
中科院分区：
文献类型：
--
作者：
Fuchsman, Clara A.;Rocap, Gabrielle
波罗的海红顶菌（Rhodopirellula baltica）、原Pirellula sp.菌株1、Blastopirellula marina、Gemmata obscuriglobus和Kuenenenia stuttgartiensis的基因组序列被用于一系列成对互惠最佳命中分析，以评估浮菌纲有争议的进化位置。与以前的报道相反，R.波罗的海有很高比例的基因与真核菌和真核菌最接近的匹配，我们在这里表明，这些浮菌与真核菌或真核菌相比，与其他细菌相比，并没有共享异常大量的基因。因此，最佳命中分析可能会分配系统发育亲和力不正确，如果近亲是从序列数据库中缺席。
The genome sequences of Rhodopirellula baltica, formerly Pirellula sp. strain 1, Blastopirellula marina, Gemmata obscuriglobus, and Kuenenia stuttgartiensis were used in a series of pairwise reciprocal best-hit analyses to evaluate the contested evolutionary position of Planctomycetes. Contrary to previous reports which suggested that R. baltica had a high percentage of genes with closest matches to Archaea and Eukarya, we show here that these Planctomycetes do not share an unusually large number of genes with the Archaea or Eukarya, compared with other Bacteria. Thus, best-hit analyses may assign phylogenetic affinities incorrectly if close relatives are absent from the sequence database.