A megaplasmid family driving dissemination of multidrug resistance in Pseudomonas
A megaplasmid family driving dissemination of multidrug resistance in Pseudomonas
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DOI：
10.1038/s41467-020-15081-7
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发表时间：
2020-03-13
影响因子：
16.6
通讯作者：
Winstanley, Craig
中科院分区：
文献类型：
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作者：
Cazares, Adrian;Moore, Matthew P.;Winstanley, Craig
多药耐药(MDR)是对健康的全球性威胁。在这里，我们使用全基因组测序来表征来自泰国一家医院的铜绿假单胞菌多药耐药临床分离株。利用长阅读序列数据，我们获得了两个紧密相关的巨质粒(>；420kb)的完整序列，它们携带着位于离散、复杂和动态耐药区域的大量抗生素耐药基因阵列，并揭示了广泛复制和重组事件的证据。综合基因组学和系统基因组学分析表明：1)这些大的质粒组成一个新出现的家族，存在于假单胞菌属的不同成员中，并与多种来源(地理、临床或环境)相关；2)巨质粒编码不同的生态位适应性附属性状，包括多药耐药；3)巨质粒家族的附属基因组高度灵活和多样化。根据我们对现有数据库的分析，巨质粒家族的历史表明，携带多重耐药基因的成员至少可以追溯到20世纪70年代。
Multidrug resistance (MDR) represents a global threat to health. Here, we used whole genome sequencing to characterise Pseudomonas aeruginosa MDR clinical isolates from a hospital in Thailand. Using long-read sequence data we obtained complete sequences of two closely related megaplasmids (>420 kb) carrying large arrays of antibiotic resistance genes located in discrete, complex and dynamic resistance regions, and revealing evidence of extensive duplication and recombination events. A comprehensive pangenomic and phylogenomic analysis indicates that: 1) these large plasmids comprise an emerging family present in different members of the Pseudomonas genus, and associated with multiple sources (geographical, clinical or environmental); 2) the megaplasmids encode diverse niche-adaptive accessory traits, including multidrug resistance; 3) the accessory genome of the megaplasmid family is highly flexible and diverse. The history of the megaplasmid family, inferred from our analysis of the available database, suggests that members carrying multiple resistance genes date back to at least the 1970s.