Degradation of synthetic dye, Rhodamine B to environmentally non-toxic products using microalgae
Degradation of synthetic dye, Rhodamine B to environmentally non-toxic products using microalgae
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DOI：
10.1016/j.colsurfb.2013.01.008
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发表时间：
2013-05-01
影响因子：
5.8
通讯作者：
Thajuddin, N.
中科院分区：
文献类型：
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作者：
Baldev, E.;MubarakAli, D.;Thajuddin, N.
在本研究中，潜在的淡水微藻Coelastrella sp。选择用于在分批培养系统中降解合成染料罗丹明 B。研究了影响脱色和降解能力的几个物理化学参数（接种浓度、初始染料浓度、温度和pH）的影响，并确定了最佳实验条件。最佳操作条件为[染料] = 100 mg l(-1)； [温度]=30℃；接种量为 10%，pH 值为 8。在这些条件下，染料在 20 天内最多实现 80% 的脱色。还测定了分离物的过氧化物酶活性，发现蛋白质含量为 2.1 mu mol min mg(-1)。通过使用各种分析技术（例如 GC-MS、TLC、FTIR 和紫外-可见光谱分析）证实了染料的实际分解。罗丹明B降解后得到小脂肪链、小链醇和酮。这些证据表明微藻在染料有效降解为无毒产品方面发挥着至关重要的作用。 (C) 2013 Elsevier B.V. 保留所有权利。
In the present study a potential freshwater microalgae Coelastrella sp. was selected for degradation of synthetic dye, Rhodamine B in batch culture system. Effect of several physico-chemical parameters that influence the decolorization followed by degradation ability was investigated (inoculum concentration, initial dye concentration, temperature and pH) and optimal experimental condition was ascertained. The optimum operating conditions were found to be [Dye] = 100 mg l(-1); [temperature] = 30 degrees C; with 10% of inoculum at a pH of 8. Under these conditions, a maximum of 80% decolorization of the dye was achieved in 20 days. Peroxidase activity of the isolate was also determined and it was found to be 2.1 mu mol min mg(-1) of protein. The actual break down of the dye was confirmed by using various analytical techniques such as GC-MS, TLC, FTIR and UV-vis spectral analysis. Small aliphatic chains, small chain alcohols and ketones were obtained after degradation of Rhodamine B. These are the evidences that showed that microalgae play a vital role on dye degradation to non-toxic products effectively. (C) 2013 Elsevier B.V. All rights reserved.