Deformation velocity imaging using optical coherence tomography and its applications to the cornea.
Deformation velocity imaging using optical coherence tomography and its applications to the cornea.
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DOI：
10.1364/boe.8.005579
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发表时间：
2017-12-01
影响因子：
3.4
通讯作者：
Zheng Y
中科院分区：
文献类型：
--
作者：
Lawman S;Madden PW;Romano V;Dong Y;Mason S;Williams BM;Kaye SB;Willoughby CE;Harding SP;Shen YC;Zheng Y
光学相干断层扫描（OCT）可以在角膜移植储存后的消肿过程中无创地监测人类供体角膜，但目前仅提取作为时间函数的最终厚度。为了可视化和量化消膨胀机制，我们提出了一种利用 OCT 数据中傅里叶相位对图像变形速度的纳米敏感性的方法。该技术通过非侵入性显示在消肿过程中通过完整上皮的渗透流可以忽略不计，并且去除内皮大约使该界面处的初始流量增加了一倍。增加的功能数据进一步验证了角膜数学模型。其中包括一种使用自动收集和半自动图形搜索分割来测量高时间分辨率（1 分钟演示）角膜厚度的有效方法。这些方法扩展了 OCT 测量组织和材料体积变化过程的能力。
Optical coherence tomography (OCT) can monitor human donor corneas non-invasively during the de-swelling process following storage for corneal transplantation, but currently only resultant thickness as a function of time is extracted. To visualize and quantify the mechanism of de-swelling, we present a method exploiting the nanometer sensitivity of the Fourier phase in OCT data to image deformation velocities. The technique was demonstrated by non-invasively showing during de-swelling that osmotic flow through an intact epithelium is negligible and removing the endothelium approximately doubled the initial flow at that interface. The increased functional data further enabled the validation of a mathematical model of the cornea. Included is an efficient method of measuring high temporal resolution (1 minute demonstrated) corneal thickness, using automated collection and semi-automated graph search segmentation. These methods expand OCT capabilities to measure volume change processes for tissues and materials.