Synthesis and biological effects of small molecule enhancers for improved recombinant protein production in plant cell cultures
Synthesis and biological effects of small molecule enhancers for improved recombinant protein production in plant cell cultures
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DOI：
10.1016/j.bioorg.2019.103452
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发表时间：
2020-01-01
影响因子：
5.1
通讯作者：
Rita Ventura, M.
中科院分区：
文献类型：
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作者：
Rebelo, Barbara A.;Santos, Rita B.;Rita Ventura, M.
组蛋白去乙酰化酶参与染色质重塑，因此在基因表达的表观遗传调控中起重要作用。HDAC抑制剂改变组蛋白和非组蛋白的乙酰化状态以调节各种细胞事件，例如转录。设计并合成了新型HDAC抑制剂以促进烟草细胞培养物中更高水平的重组蛋白生产。这些化学增强剂对植物细胞表观遗传谱的影响已通过分子对接、体外和体内研究进行了评估。这些新的增强子的添加导致组蛋白H3乙酰化水平的增加，这促进了细胞培养物中重组蛋白的积累水平的增加。这些结果可以为这些增强子的应用铺平道路，以提高植物细胞为基础的系统中的高价值产品的生产。
Histone deacetylases are involved in chromatin remodelling and thus play a vital role in the epigenetic regulation of gene expression. HDAC inhibitors alter the acetylation status of histone and non-histone proteins to regulate various cellular events such as transcription. Novel HDAC inhibitors were designed and synthesised to promote higher levels of recombinant protein production in tobacco cell cultures. The effect of these chemical enhancers on the epigenetic profiles in plant cells has been evaluated by molecular docking, in vitro and in vivo studies. The addition of these novel enhancers led to an increase in histone H3 acetylation levels that promoted an increase in the accumulation levels of the recombinant protein in cell culture. These results can pave the way for the application of these enhancers to improve the production of high value products in plant cell based systems.