RELATIONSHIP BETWEEN VIRULENCE VARIATION AND DNA POLYMORPHISM IN PUCCINIA-STRIIFORMIS
RELATIONSHIP BETWEEN VIRULENCE VARIATION AND DNA POLYMORPHISM IN PUCCINIA-STRIIFORMIS
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DOI：
10.1094/phyto-83-1489
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发表时间：
1993-12-01
期刊：
影响因子：
3.2
通讯作者：
LEUNG, H
中科院分区：
文献类型：
--
作者：
CHEN, XM;LINE, RF;LEUNG, H
对23个小麦条锈菌（Pucciniastriiformis f.）选择代表北美不同地区和不同毒力模式的小麦条锈病（stripe rust of wheat），分析毒力和DNA多态性的变化。毒力表征是基于15个小麦品种上的侵染类型的表达，用于区分北美条锈菌小种。在23个收集物中，20个具有一致不同的毒力模式，即，这20个收集物中的每一个都是不同的小种，并且每个收集物的所有5个分离物都具有相同的毒力模式。两个集合由两种毒力模式的分离株组成，一个集合由三种毒力模式的分离株组成。在26个毒力型（小种）中，有4个（命名为小种CDL-47、CDL-48、CDL-49和CDL-50）以前未检测到。随机扩增多态性DNA（RAPD）分析DNA多态性。使用随机引物对从115个分离株中提取的DNA进行扩增。11个随机引物共扩增出107条带，其中多态性条带占47%。DNA多态性在小种间和小种内的单孢菌株间均存在。来自加州小黑麦的小种CDL-21在13%的RAPD带型中与其它小种不同，与其它小种的相似性仅为67%。所有其他种族更密切相关（约80%的相似性）。聚类分析结果表明，115个菌株可分为6个毒力类群和5个RAPD类群。致病力与RAPD相似性矩阵之间的相关系数较低（0.20）。基于RAPD数据的聚类分析将对条锈抗性基因Yr 1有毒性的菌株与对Yr 1无毒性的菌株区分开来。毒力类群与流行区域高度相关。RAPD组一般不与地理区域。毒力和RAPD模式之间的低关联表明，DNA多态性是独立的毒力，和选择的毒力越来越多的主机与种族特异性的电阻起着重要作用，在确定种族结构的病原体。
One-hundred, fifteen single-urediospore isolates from 23 collections (five isolates per collection) of Puccinia striiformis f. sp. tritici (stripe rust of wheat) selected to represent different regions in North America and different virulence patterns were analyzed for variation in virulence and DNA polymorphism. Virulence characterization was based on the expression of infection types on 15 wheat cultivars used to differentiate races of P. striiformis in North America. Of the 23 collections, 20 had uniformly different virulence patterns, i.e., each of the 20 collections was a different race, and all five isolates of each collection had the same virulence pattern. Two collections consisted of isolates with two virulence patterns, and one collection consisted of isolates with three virulence patterns. Of the 26 virulence patterns (races), four (designated as races CDL-47, CDL-48, CDL-49, and CDL-50) had not been detected previously. DNA poly morphism was determined by random amplified polymorphic DNA (RAPD) assay. Random primers were used to amplify DNA extracted from the 115 isolates. Of 107 RAPD bands that were amplified with 11 random primers, 47% were polymorphic. DNA polymorphism was detected among races and among single-spore isolates within races. Race CDL-21, originally collected from triticale in California, was different from other races in 13% of the RAPD bands and had only 67% similarity with other races. All other races were more closely related (about 80% similarity). Based on cluster analyses, the 115 isolates were placed in six virulence and five RAPD groups. The correlation coefficient between the virulence and RAPD similarity matrices was low (0.20). Cluster analysis based on RAPD data separated isolates virulent on stripe rust-resistance gene Yr1 from those avirulent on Yr1. Virulence groups were highly associated with epidemic regions. RAPD groups generally were not associated with geographic regions. The low association between virulence and RAPD patterns indicates that DNA polymorphisms are independent of virulence, and selection for virulence by growing hosts with race-specific resistance plays a major role in determining the race structure of the pathogen.