hsa_circRNA_101237: A Novel Diagnostic and Prognostic Biomarker and Potential Therapeutic Target for Multiple Myeloma
hsa_circRNA_101237: A Novel Diagnostic and Prognostic Biomarker and Potential Therapeutic Target for Multiple Myeloma
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DOI：
10.2147/cmar.s241089
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Wang, Xiang
中科院分区：
文献类型：
--
作者：
Liu, Xiao;Tang, Hao;Wang, Xiang
背景资料：研究表明，环状RNA（circular RNA，circRNA）在肿瘤的发生发展中起着至关重要的作用，但大多数circRNA对肿瘤患者的诊断和预测价值尚不清楚，尤其是对多发性骨髓瘤（multiple myeloma，MM）的诊断和预测价值。采用高通量circRNA芯片测序技术鉴定MM中差异表达的circRNA，qRT-PCR检测hsa_circRNA_101237在来自143名MM患者（65名首次发作未接受过治疗的患者和78名复发性/难治性疾病患者）、MM细胞和硼替佐米耐药MM细胞系的骨髓组织中的表达水平。探讨hsa_circRNA_101237是否可作为MM的潜在生物标志物和治疗靶点。结果：hsa_circRNA_101237在MM患者（尤其是复发/难治性疾病患者）骨髓组织、MM细胞和硼替佐米耐药MM细胞系中的平均表达显著增加（P
Background: It has been demonstrated that circular RNA (circRNA) plays a crucial role in the occurrence and development of tumors, but the diagnostic and predictive value of most circRNAs in tumor patients remains unclear, especially for multiple myeloma (MM).Methods: High-throughput circRNA microarray-based sequencing was used to identify the differentially expressed circRNAs in MM. qRT-PCR was then employed to detect hsa_circRNA_101237 expression levels in the bone marrow tissues from 143 MM patients (65 first-episode treatment-naive patients and 78 patients with recurrent/refractory disease), MM cells and bortezomib-resistant MM cell lines. Whether hsa_circRNA_101237 can be used as a potential biomarker and therapeutic target for MM was investigated.Results: The average expressions of hsa_circRNA_101237 in the bone marrow tissues from MM patients (especially those with recurrent/refractory disease), MM cells and bortezomib-resistant MM cell lines were increased significantly (P