AMINOPYRINE N-DEMETHYLASE ACTIVITY IN HUMAN LIVER-MICROSOMES
AMINOPYRINE N-DEMETHYLASE ACTIVITY IN HUMAN LIVER-MICROSOMES
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DOI：
10.1038/clpt.1990.184
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发表时间：
1990-11-01
影响因子：
6.7
通讯作者：
BENITEZ, J
中科院分区：
文献类型：
--
作者：
GARCIAAGUNDEZ, JA;LUENGO, A;BENITEZ, J
本文研究了31例不同病人肝微粒体氨基比林N-脱甲基酶活性、细胞色素P-450、细胞色素b5和NADPH还原酶活性。我们的研究结果表明，存在显着的个体间，但没有性别或年龄相关的差异，N-脱甲基酶活性（范围在0.52和4.42 nmol/min/mg蛋白质），这些差异与细胞色素P-450和NADPH还原酶活性的含量直接相关，但不与细胞色素b5含量，在样品中。相反，在氨基比林（平均值，2.4 mmol/L）或NADPH（平均值，69 μ mol/L）的Michaelis-Menten常数值中没有发现差异，强烈地表明个体间差异可能是由于在所研究的群体中出现不同量的相同酶，而不是不同酶的存在。
Aminopyrine N-demethylase activity and the contents of cytochrome P-450, cytochrome b5, and NADPH-reductase activity in human liver microsomes from 31 different patients were studied. Our results show the existence of significant interindividual, but not sex- or age-related differences in N-demethylase activity (ranging between 0.52 and 4.42 nmol/min/mg protein), and that these differences are directly correlated with the content of the cytochrome P-450 and NADPH-reductase activity, but not with that of cytochrome b5 content, in the samples. In contrast no differences were found in the Michaelis-Menten constant values for aminopyrine (mean, 2.4 mmol/L) or NADPH (mean, 69 .mu.mol/L), strongly suggesting that interindividual differences could be due to the occurrence of different amount of the same enzyme, rather than the presence of different enzymes, in the population studied.