Impact of storage solution formulation during refrigerated storage upon chondrocyte viability and cartilage matrix.
Impact of storage solution formulation during refrigerated storage upon chondrocyte viability and cartilage matrix.
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冷藏期间储存溶液配方对软骨细胞活力和软骨基质的影响。
DOI：
10.1159/000363134
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yao,Hai
中科院分区：
文献类型：
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作者：
Wright,GregoryJ;Brockbank,KelvinGM;Rahn,Eliza;Halwani,DinaO;Chen,Zhen;Yao,Hai
各种保存方法已被评估用于组织移植的更长时间的低温软骨储存；然而，结果喜忧参半。本研究旨在确定在软骨储存临床使用的时间内，磷酸盐缓冲盐水（PBS）或器官保存液是否能比培养液更好地保存关节软骨的细胞外基质和软骨细胞。将不含骨的猪软骨塞分别在含胎牛血清（FBS）、PBS、Belzer’s和Unisol溶液和不含胎牛血清的培养基中保存1个月，温度为4℃。使用reazurin还原法检测代谢活性，并通过测量电导率评估基质渗透性。在含10% FBS的培养基中保存7天，软骨代谢功能良好，保存1个月后，软骨代谢功能降至36%左右。保存1个月后，添加和不添加FBS的培养基中样品的存活率无显著差异（p= 0.5005）。在PBS和两种不同的溶液（Belzer's和Unisol）中冷藏软骨，设计用于最佳冷藏组织和器官储存，导致软骨细胞功能丧失和基质通透性保留。相反，在培养基中观察到相反的情况，即软骨细胞功能的保留和基质通透性的丧失。未来的研究重点应放在通过储液配方将软骨细胞功能的保留与基质通透性结合起来。
Various preservation solutions have been evaluated for longer hypothermic cartilage storage for tissue transplantation; however, the results are mixed. This research was carried out to determine whether phosphate-buffered saline (PBS) or organ preservation solutions would preserve both the extracellular matrix and chondrocytes of articular cartilage better than culture medium during refrigerated storage in the time frame that cartilage is stored for clinical use. Porcine cartilage plugs were stored, without the underlying bone, in culture medium with and without fetal bovine serum (FBS), PBS, Belzer's and Unisol solutions for 1 month at 4 C. Metabolic activity was tested using a resazurin reduction method, and matrix permeability was evaluated by measuring electrical conductivity. Storage in culture medium with 10% FBS was shown to provide good cartilage metabolic function for 7 days, decreasing to about 36% after 1 month of storage. There was no significant difference between samples stored in culture medium with and without FBS after 1 month of storage (p= 0.5005). Refrigerated storage of cartilage in PBS and two different solutions (Belzer's and Unisol) designed for optimal refrigerated tissue and organ storage results in loss of chondrocyte function and retention of matrix permeability. In contrast, the opposite, namely significantly better retention of chondrocyte function and loss of matrix permeability, was observed with culture medium. Future research should be focused on combining retention of chondrocyte function and matrix permeability by storage solution formulation.