SYNTHESIS OF AN OXYAMIDE LINKED NUCLEOTIDE DIMER AND INCORPORATION INTO ANTISENSE OLIGONUCLEOTIDE SEQUENCES
SYNTHESIS OF AN OXYAMIDE LINKED NUCLEOTIDE DIMER AND INCORPORATION INTO ANTISENSE OLIGONUCLEOTIDE SEQUENCES
复制标题
DOI：
10.1039/c39940000915
复制
发表时间：
1994-04-21
影响因子：
--
通讯作者：
RAGHAVACHARI, R
中科院分区：
文献类型：
--
作者：
BURGESS, K;GIBBS, RA;RAGHAVACHARI, R
制备了两种胸苷衍生物，即5 '-保护的3'-羟胺和5 '-羧基-3'-保护的化合物，并将其偶联以形成氧酰胺连接的二核苷酸类似物;将该二聚体掺入寡脱氧核苷酸中，然后显示出与互补DNA退火，其亲和力与天然序列几乎相同。
Two derivatives of thymidine, a 5'-protected 3'-hydroxylamine and 5'-carboxy-3'-protected compound, were prepared and coupled to form an oxyamide-linked dinucleotide analogue; this dimer was incorporated into an oligodeoxynucleotide then shown to anneal to complementary DNA with nearly the same affinity as the natural sequence.