Function and Immunocytochemical Localization of Two Novel Odorant-Binding Proteins in Olfactory Sensilla of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)
Function and Immunocytochemical Localization of Two Novel Odorant-Binding Proteins in Olfactory Sensilla of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)
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圣甲虫Holotrichia oblita Faldermann（鞘翅目：金龟科）嗅觉感受器中两种新型气味结合蛋白的功能和免疫细胞化学定位
DOI：
10.1093/chemse/bjr084
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发表时间：
2012-02-01
期刊：
影响因子：
3.5
通讯作者：
Li, Kebin
中科院分区：
文献类型：
--
作者：
Deng, Sisi;Yin, Jiao;Li, Kebin
气味结合蛋白（OBP）在昆虫和脊椎动物中都有发现，据信它们参与化学通讯。在这项研究中，我们鉴定和表达2 OBP金龟子，Holotrichia oblita Faldermann（鞘翅目：金龟子科）。HoblOBP 1与其他金龟子OBP显示出更多的相似性，而HoblOBP 2则更多样化。以N-苯基-1-萘胺（1-NPN）为荧光探针进行配体结合实验，结果表明两种HoblOBP都喜欢植物挥发物而不喜欢H.性信息素Hob 10 BP 1显示对更宽范围的测试化合物的结合亲和力，但Hob 10 BP 2显示更特异的结合亲和力。肉桂醛和2，4-二叔丁基苯酚与Hob 1 OBP 1结合后，能引起雌蛾触角的强烈电生理反应。oblita成年人，分别。水杨酸甲酯也显示出对Hob 10 BP 2的良好亲和力，并且它可以引起适度的电生理反应。虽然β-紫罗兰酮是结合力最强的配体之一，但它引起的电生理反应较弱。在免疫细胞化学分析中，我们观察到HoblOBP 1和HoblOBP 2在两性的锥形感器和板状感器中共表达。
Odorant-binding proteins (OBPs) are found in both insects and vertebrates, and it is believed that they are involved in chemical communication. In this study, we identify and express 2 OBPs from the scarab beetle, Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae). HoblOBP1 shows more similarities with other scarab beetle OBPs, whereas HoblOBP2 is more diverse. N-phenyl-1-naphthylamine (1-NPN) is used as a fluorescent probe in ligand-binding experiment, and results indicate that both HoblOBPs prefer plant volatiles to putative H. oblita sex pheromones. HoblOBP1 shows binding affinity to a wider range of test compounds, but HoblOBP2 displays more specific binding affinity. Cinnamaldehyde and 2,4-di-tert-butylphenol bind to HoblOBP1 can elicit strong electrophysiological responses of the antennae from female H. oblita adults, respectively. Methyl salicylate also shows good affinity to HoblOBP2 and it can elicit moderate electrophysiological responses. Although, beta-ionone is one of the ligands of the strongest binding, it elicits a weak electrophysiological response. In the immunocytochemical analysis, we observe that HoblOBP1 and HoblOBP2 are coexpressed in sensilla basiconica and placodea in both sexes.