Muscarinic acetylcholine receptors in chick heart: influence of heat and N-ethylmaleimide on receptor conformations and interactions with guanine nucleotide-dependent regulatory proteins.
Muscarinic acetylcholine receptors in chick heart: influence of heat and N-ethylmaleimide on receptor conformations and interactions with guanine nucleotide-dependent regulatory proteins.
复制标题
鸡心中的毒蕈碱乙酰胆碱受体：热和 N-乙基马来酰亚胺对受体构象的影响以及与鸟嘌呤核苷酸依赖性调节蛋白的相互作用。
DOI：
10.1016/s0304-3940(85)80093-8
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发表时间：
1985
影响因子：
2.5
通讯作者：
Smith,MD
中科院分区：
文献类型：
--
作者：
Aronstam,RS;Kirby,ML;Smith,MD
Several conformations of muscarinic acetylcholine receptors in chick heart are revealed by the energetics of agonist binding. Heating cardiac membranes at 50°C for 5 min decreased agonist affinity, steepened agonist binding curves and eliminated receptor sensitivity to guanine nucleotides. N-ethylmaleimide (NEM) steepened agonist binding curves and eliminated receptor sensitivity to guanine nucleotides without decreasing agonist binding affinity. NEM prevented and reversed the effect of heat on agonist affinity. Pirenzepine and N-methylscopolamine binding were unaffected by either treatment. NEM and heat stabilize muscarinic receptors in conformations unrelated to those associated with naturally occurring receptor populations.
