Epimedium polysaccharide alleviates polyglutamine-induced neurotoxicity in Caenorhabditis elegans by reducing oxidative stress
Epimedium polysaccharide alleviates polyglutamine-induced neurotoxicity in Caenorhabditis elegans by reducing oxidative stress
复制标题
淫羊藿多糖通过减少氧化应激减轻多聚谷氨酰胺诱导的秀丽隐杆线虫神经毒性
DOI：
10.1089/rej.2016.1830
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发表时间：
2017
影响因子：
2.6
通讯作者：
Zebo Huang
中科院分区：
文献类型：
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作者：
Yanxia Xiang;Ju Zhang;Haifeng Li;Qiangqiang Wang;Lingyun Xiao;Hu;i Weng;Xiaobin Zhou;Chung Wah Ma;Fangli Ma;Minghua Hu;Zebo Huang
淫羊藿传统上被用于治疗各种疾病，包括神经系统疾病。本研究从短荆芥中分离出一种酸性多糖EbPS-A1，主要含有半乳糖醛酸、半乳糖和鼠李糖，还含有阿拉伯糖和葡萄糖醛酸。通过秀丽隐杆线虫模型，我们发现EbPS-A1能够抑制由多聚谷氨酰胺（polyQ）介导的行为功能障碍，多聚谷氨酰胺与亨廷顿氏病等几种神经退行性疾病有关。有趣的是，EbPS-A1不会抑制polyQ聚集或延长线虫的寿命；然而，它确实提高了被百草枯中毒的多q型和野生型线虫在增加氧化应激下的存活率。进一步的研究表明，EbPS-A1不仅具有体外清除自由基的能力，还具有降低活性氧水平、增强抗氧化酶活性、降低脂质过氧化产物的能力。综上所述，这些结果表明，对多q介导的神经毒性的保护作用可能是由于其抗氧化功能。
Epimediumhas been traditionally used to treat a variety of medical conditions, including neurological disorders. In this study, an acidic polysaccharide EbPS-A1 is isolated fromEpimedium brevicornumand found to contain mainly galacturonic acid, galactose, and rhamnose but also arabinose and glucuronic acid. UsingCaenorhabditis elegansmodels, we show that EbPS-A1 is capable of inhibiting behavioral dysfunction mediated by polyglutamine (polyQ), which is implicated in several neurodegenerative disorders such as Huntington's disease. Interestingly, EbPS-A1 does not inhibit polyQ aggregation or extend lifespan in the nematodes; it does, however, improve the survival under increased oxidative stress of both polyQ and wild-type nematodes intoxicated by paraquat. Further studies reveal that EbPS-A1 is capable of not only scavenging free radicalsin vitrobut also reducing reactive oxygen species levels, enhancing antioxidant enzyme activities, and decreasing lipid peroxidation product inC. elegansmodels. Together, these results suggest that the protective effect ofEpimediumpolysaccharide against polyQ-mediated neurotoxicity is likely due to its antioxidant function.