The relationship between hetero-oligomer formation and function of the topological specificity domain of the Escherichia coli MinE protein.
The relationship between hetero-oligomer formation and function of the topological specificity domain of the Escherichia coli MinE protein.
复制标题
异源寡聚物形成与大肠杆菌 MinE 蛋白拓扑特异性结构域功能之间的关系。
DOI：
10.1046/j.1365-2958.1998.01059.x
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发表时间：
1998
影响因子：
3.6
通讯作者：
Rothfield,L
中科院分区：
文献类型：
--
作者：
Zhang,Y;Rowland,S;King,G;Braswell,E;Rothfield,L
MinE是一种寡聚蛋白，与其他Min蛋白一起，是大肠杆菌细胞分裂位点的适当放置所必需的。我们通过分析超离心和非变性聚丙烯酰胺凝胶中异质低聚物形成的研究，研究了MinE的自结合特性。纯化矿的自缔合性质预示着胞质矿很可能以单体和二聚体的混合物形式存在。与这一预测一致，当蛋白共表达时，C端mine22 - 88片段与MinE+形成异聚物。相比之下，mine36 - 88片段不会形成MinE+/ mine36 - 88异聚物，尽管在野生型细胞中，当mine36 - 88与MinE+共表达时，其诱导小细胞形成的能力表明，mine36 - 88会影响隔膜放置的拓扑特异性。因此，在野生型细胞中，通过表达mine36 - 88诱导迷你化，异齐聚物的形成并不是必需的。对正常间隔放置的干扰归因于mine36 - 88与完整MinE蛋白中相应结构域之间对间隔放置拓扑特异性所需成分的竞争。
MinE is an oligomeric protein that, in conjunction with other Min proteins, is required for the proper placement of the cell division site ofEscherichia coli. We have examined the self‐association properties of MinE by analytical ultracentrifugation and by studies of hetero‐oligomer formation in non‐denaturing polyacrylamide gels. The self‐association properties of purified MinE predict that cytoplasmic MinE is likely to exist as a mixture of monomers and dimers. Consistent with this prediction, the C‐terminal MinE22–88fragment forms hetero‐oligomers with MinE+when the proteins are co‐expressed. In contrast, the MinE36–88fragment does not form MinE+/MinE36–88hetero‐oligomers, although MinE36–88affects the topological specificity of septum placement as shown by its ability to induce minicell formation when co‐expressed with MinE+in wild‐type cells. Therefore, hetero‐oligomer formation is not necessary for the induction of minicelling by expression of MinE36–88in wild‐type cells. The interference with normal septal placement is ascribed to competition between MinE36–88and the corresponding domain in the complete MinE protein for a component required for the topological specificity of septal placement.